Mung bean from seedlings

Some members of the Liliaceae accumulate free azetidine-2-carboxylic acid in a much higher concentration than that found to be lethal to mung bean seedlings, but it is not incorporated into their proteins. Fowden (43) postulated that these plants either had a proline-incorporating system which was more specific than that found in other species, or some subcellular mechanism operated to prevent the homolog from reaching the sites involved in protein synthesis. Data which supported the first suggestion were subsequently obtained (116). 

Mizutani M, Ohta D, Sato R (1993) Purification and characterization of a cytochrome P450 (rran -cinnamic acid 4-hydroxylase) from etiolated mung bean seedlings. Plant Cell Physiol 34 481-488... 

Conversion of the a-D-glucopyranosyl derivative (94a) into the a-D-galactopyranosyl ester (95a) was demonstrated370 in 1951 as the first example of an enzymic reaction of a sugar nucleotide. The enzyme that catalyzes this reaction, namely, uridine 5 -(a-D-glu-copyranosyl pyrophosphate) 4"-epimerase,371 is common in Nature. Purified preparations have been obtained from yeast,372 373 Escherichia coli 374-376 mung-bean seedlings,377 wheat germ,378 and animal tissues.244,379 380... 

An enzyme similar to the 3 -nucleotidase of mung bean has been isolated from germinating wheat seedlings and purified 800-fold (90). The preparation possessed DNase, RNase, and 3 -nucleotidase activities. These three activities were similar in pH optima, requirements for Zn2+ and sulfhydryl compounds, stability to storage, temperature inactivation... 

The conversion of D-mannose 6-phosphate into D-mannosyl phosphate is catalyzed by phosphomannomutase, an enzyme distinct from phosphoglucomutase. Both enzymes have been detected by Mathe-son705 in cassia seeds, mung beans, orchid tubers, and pea seedlings. These enzymes from cassia seeds have been separated from one another by chromatography on DEAE- and O-phosphono-cellulose columns, and further characterized. Phosphomannomutase from animal sources requires the presence of either D-galactose 1,6-bisphosphate or D-mannose 1,6-bisphosphate for activity.708 D-Mannosyl phosphate may then be enzymically transformed into GDP-D-mannose in the... 

The interaction of F with Ca has been widely known. Many enzymes that occur in different plant tissues have been shown to require Ca for activity. Examples include amylase (Yu 1986) and invertase (Yu 1997 Ouchi et al. 1999) from germinating mung bean seedlings. 

Rehbock, B., Ganber, D., and Berger, R.G., Efficient generation of 2E-hexenal by a hydroperoxide lyase from mung bean seedlings, Food Chem., 63, 161, 1998. 

Cytochrome 6-560 isolated and purified from mung bean seedlings exists in mitochondria of the organism and corresponds functionally to cytochrome 6 in animal mitochondria in that it shows similar spectral properties and standard potential 266). Bonner has suggested that cytochrome 6-560 is identical to cytochrome which was found in mitochondria from the spadix of Arum maculatum 268). The Eo of cytochrome 67 269,270) was found to be at —30 mV. Both cytochromes... 

MIZUTANI, M., OHTA, D., SATO, R., Purification and Characterization of a Cytochrome-P450 (Trans-Cinnamic Acid 4-Hydroxylase) from Etiolated Mung Bean Seedlings, Plant Cell Physiol., 1993, 34, 481-488. 

Metal requirements for the plant enzyme closely resemble those of the microbial enzyme which require a divalent cation such as Mn for full activity. Thus, Mn " and Co activated the synthase from pea seedlings and cauliflower florets (Rothe et al., 1976 Huisman and Kosuge, 1974) but Mg " " was the most effective activator for the mung bean enzyme (Minamikawa, 1967 Minamikawa and Uritani, 1967). Three multiple forms of the enzyme were detected in pea seedlings but little is known about their intracellular... 

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