Multiscreen filtration

Aqueous solubility values for the samples analyzed compared favorably with results obtained by traditional methods. The solubility values for amiodarone HC1, reserpine, and benzanthrone were lower than the LOQ of the, uPLC system used for the evaluation. Results of the evaluation of compound solubility employing no-filtration /iPLC were compared with those obtained by two traditional methods (1) multiscreen filtration followed by a UV plate reader, and (2) the shake flask method followed by a UV plate reader. As shown in Figure 6.31, the solubility values determined by the different methods are comparable for most compounds examined. Figure 6.32 shows the results of evaluations of aqueous solubility at four different pH levels for phenazopyridine and piroxicam samples. 

FIGURE 6.31 Comparison of solubility results obtained by no-filtration method followed by PLC detection and those obtained by two traditional methods multiscreen filtration followed by UV plate reader, and shake flask method followed by UV plate reader. (Data provided by Steven Hobbs, Courtney Coyne, and Gregory Kazan.)... 

Vacuum filtration at 18 to 20 in. Hg was performed on a Multiscreen HTS vacuum manifold with a deep well collar. For the SPE, the extraction plate was conditioned first with 100 /.iL of methanol... 

Vacuum filtration apparatus for separating free and bound radioligand in 96-well filtration plates (Millipore MultiScreen System or equivalent). 

The inhibiting activity of selected experimental agents was determined through a method of assay based on the use of the MultiScreen-PH 96-well plate in which phosphocellulose filter paper was placed at the bottom of each well allowing binding of positive charged substrate after a washing/filtration step. 

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