Multiplicative ambiguity analyses

Unfortunately, the utility of this method for many of the more interesting enzymes is restricted by the complexity of the protein. If more than one type of cluster is present, the multiple component analysis on the extruded mixture may lead to ambiguous conclusions. In addition non-metallochromophores may interfere. Holm and co-workers (Wong et ai, 1979) circumvented some of these problems for Fe S proteins by their choice of spectral analysis and exogenous thiolate ligand. Namely, they used F NMR spectroscopy to analyze the products of thiolate extrusion with /)-trifluoromethylbenzenethiol. Contact shifts for the fluorine resonances are considerably different for 2Fe and 4Fe clusters. Important restrictions on the use of the F NMR detection are the quantity of protein needed, the synthesis of the ligand, and access to the spectrometer. 

With the development of new liquid phases, interest in the separation of alditol acetates has revived, and this now may be considered the most widely used method for analyzing carbohydrate mixtures, provided that reduction does not introduce an ambiguity into the analysis also, it must be borne in mind that the multiplicity of peaks obtained by trimethylsilylation of a free sugar or of its methyl glycosides may serve a useful purpose in characterization. 

This paper is based on a qualitative textual analysis. Texts can be one point of departure to deal with realities that are ambiguous and multiple, and uncover some of the complexities of social phenomena (Law 2004). Textual investigations, however, can be performed in several ways and on different types of documents such as statistical records, files, images and texts (Silverman 1993). 

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