Multiple cerebrospinal fluid analysis

R6. Reiber, H., Cerebrospinal fluid analysis. In CSF Analysis in Multiple Sclerosis (E. J. Thomson, M. Trojano, and R Livrea, eds.). Springer-Verlag, Milano, 1996. 

Cerebrospinal Fluid Analysis in Multiple Sclerosis Francisco A. Luque and Stephen L. Jaffe... 

Hammack BN, Eung KY, Hunsucker SW, Duncan MW, Burgoon MP, Owens GP, Gilden DH (2004) Proteomic analysis of multiple sclerosis cerebrospinal fluid. Mult Scler 10 245-260. 

The main clinical significance of cerebrospinal fluid (CSF) protein eleetrophoresis is for the detection of the oligoclonal bands, which are present in multiple sclerosis in the gamma region. Similar to urine, proteins in the CSF are present fluid in very low concentration (100 times less than serum). For the majority of the samples, a 10- to 20-fold concentration is preferred before analysis by CE (by the same membrane concentrators used for urine). CSF protein separation can be accomplished in less than 10 min with CE versus 2 h for AG with the ability to detect oligoclonal banding by this technique [36]. 

Unlike mass spectroscopy, gel electrophoresis does not provide a quantitative value for the amount of given protein. However, it provides a low cost and relatively rapid method for the analysis of multiple proteins in a specimen, especially when implemented as a capillary electrophoresis system. Therefore, it has been used for the separation of enzymes (e.g., creatinine phosphokinase), mucopolysaccharides, plasma, serum, cerebrospinal fluid, urine, and other bodily fluids [13]. It is also used for quality control applications for the manufacturing of biological compounds to verify the purity or to examine the manufacturing yield [14]. 

R41 N. W. Lutz and P. J. Cozzone, Metabolic Profiling in Multiple Sclerosis and Other Disorders by Quantitative Analysis of Cerebrospinal Fluid Using Nuclear Magnetic Resonance Spectroscopy , Curr. Pharm. Biotechnol., 2011, 12, 1016. 

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