Multidimensional hquid chromatography

Di Pahna, S., Hennrich, M. L., Heck, A. J. R., Mohammed, S. (2012). Recent advances in peptide separation by multidimensional hquid chromatography for proteome analysis. Journal of Proteomics, 75, 3791-3813. 

Valentine, S.J., Kulchania, M., Srebalus Barnes, C.A., Clemmer, D.E. (2001). Multidimensional separations of complex peptide mixtures a combined high-performance hquid chromatography/ion mobility/time-of-flight mass spectrometry approach. Int. J. Mass Spectrom. 212, 97-109. 

The use of essential oils is increasing because of the increase in the number of their apphcations and in the framework of natural and environmentally friendly materials. Many times the analysis of their components is quite complex due to the high number and the diversity of compounds in their composition. In this entry a general overview of the extraction methods is given by comparing conventional hquid-liquid and sohd-hquid methods with new alternative ones, such as supercritical fluid extraction and microwave-assisted extraction. Gas chromatography methods and examples are treated and important issues such as detection systems, modem hbraries for compounds identification, as well as multidimensional or hyphenated techniques are discussed. The use of these modem techniques and methods has improved resolution and sensitivity in essential oils determination and could open the possibihty of future work in this area of chromatography. 

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