Multicolor imaging

Different substituents on the carboxy-functionalized fluorescein can be introduced to produce marked alterations in the absorbance and fluorescence emission wavelengths, as well as in other physical properties. The selective substitution of chlorine for aromatic hydrogen has been found to increase fluorescence efficiency and to narrow emission and absorbance maxima when compared with fluorescein 48, which is useful in multicolor imaging. 

The relatively nonpolar squaraine rotaxane 14c was found to interact with cells in a very similar way to the well-known lipophilic dye Nile Red this probe rapidly accumulates at lipophilic sites inside a living cell, such as the endoplasmic reticulum and intracellular lipid droplets [55], The red emission band for probe 14c is quite narrow and permits the acquisition of multicolor images. It displayed high chemical stability and low toxicity. 

Bertera S, Geng X, Tawdrous Z, Bottino R, Balamurugan AN, Rudert WA, Drain P, Watkins SC, Trucco M. Body window-enabled in vivo multicolor imaging of transplanted mouse islets expressing an insulin-dimer fusion protein. Biotechniques 2003, 35, 718-722. 

L. Schermelleh, P.M. Carlton, S. Haase, L. Shao, L. Winoto, P. Kner, B. Burke, M.C. Cardoso, D.A. Agard, M.G.L. Gustafsson, et ah. Subdiffraction multicolor imaging of the nuclear periphery with 3D structured illumination microscopy. Science 320, 1332-1336 (2008)... 

Fig. 6. Multicolor imaging of protein expression patterns of Staphylococcus aureus COL under anaerobic conditions in the presence and absence of nitrate. Delta2D software is used to visualize complex protein expression patterns on the 2D image in the standard pH range 4-7. The color code is represented on the right side. See Color Plate 4, following p. 46.)...

Sonnichsen, B., De Renzis, S., Nielsen, E., Rietdorf, J., and Zerial, M. (2000). Distinct membrane domains on endosomes in the recycling pathway visualized by multicolor imaging of Rab4, Rab5, and Rabll. J. Cell Biol 149, Wl-914. 

The success of FISH relies upon photostability, narrow emission spectral characteristics as well as multicolor imaging capabilities. Qdots appear to be an ideal candidate for... 

For living-cell microscopy, multilabeling acquisition is constrained by the fact that objects are likely to move during the collection of stacks. Multicolor images must therefore be acquired as rapidly as possible, and the sequential stack-color mode described above is inappropriate. There is a single solution to this problem using a multiband pass dichroic mirror and dissociating... 

L. Urano, Y. Multicolor imaging of lymphatic function with two nanomaterials quantum dot-labeled cancer cells and dendrimer-based optical agents. Nanomedicine 2009,4,411 19. 

The process of transforming a multicolor original into an accurate and pleasing color reproduction involves a series of complex manipulations conversion of continuous tones into halftones separation of multicolor images into individual films for the three primary colors and black and adjustment of color balance and composition. Prepress color proofing systems make it possible to check accuracy of these transformations without the high cost and time delay of on-press proofing. 

U.S. 4,356,253. Photoimaging Process for Forming Multicolor Images. Buzzell, John G. (E. I. Du... 

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