Multianalyte array biosensor

Seo et al. (1999) used a planar optic biosensor that measures the phase shift variation in refractive index due to antigen binding to antibody. In this method, they were able to detect S. enterica serovar T) himurium with a detection limit of 1 x 10 cfu/ml. When chicken carcass fluid was inoculated with 20 cfu/ml, the sensor was able to detect this pathogen after 12 h of nonselective enrichment. A compact fiber optic sensor was also used for detection of S. T) himurium at a detection limit of 1 X 10" cfu/ml (Zhou et al., 1997, 1998) however, its efficacy with food samples is unproven. Later, Kramer and Lim (2004) used the fiber optic sensor, RAPTOR , to detect this pathogen from spent irrigation water for alfalfa sprouts. They showed that the system can be used to detect Salmonella spiked at 50 cfu/g seeds. An evanescent wave-based multianalyte array biosensor (MAAB) was also employed for successful testing of chicken excreta and various food samples (sausage, cantaloupe, egg, sprout, and chicken carcass) for S. T) himurium (Taitt et ah, 2004). While some samples exhibited interference with the assay, overall, the detection limit for this system was reported to be 8 x 10 cfu/g. 

Taitt CR, Golden JP, Shubin YS et al (2004) A portable array biosensor for detecting multianalytes in complex samples. Microb Ecol 47 175-185... 

E. J. Cho, Z. Tao, E. C. Tehan and F. V. Bright, Multianalyte pin-printed biosensor arrays based on protein-doped xerogels, Anal. Chem., 2002, 74, 6177. 

In addition to the two-biosensor examples outlined above, protein arrays are the most heavily studied of the multianalyte immunobiosensing platforms and, therefore. 

Cho, E.J., et al.. Multianalyte Pin-Printed Biosensor Arrays Based on Protein-Doped Xerogels. Analytical Chemistry, 2002. 74 p. 6177-6184. 

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