Mueller-Hinton agar

Note 2 Fungi spores from an active culture were suspended in 1 ml sterile 0.85 NaCl, then lawned on 150 mm Mueller-Hinton agar plates with a sterile cotton swab... 

These included nutrient agar (Oxoid), Mueller-Hinton Agar (Oxoid), Desoxy-cholate Citrate Agar (Oxoid), and MacConkey Agar (Oxoid). 

Micrococcus luteus (ATCC 9341) Assay. Mueller-Hinton agar plates were overlayed with 0.7% Difco Bacto-agar containing approximately 2.5 x 10 cells of M. luteus/ml. When the agar is set, 15 yl of... 

The determination of MIC of antibiotics was performed by microtiter method. Antimicrobial agents were diluted by serial dilutions MPB in flat-bottomed plates, they were also added to the culture of Staphylococcus aureus, and were incubated for 24 hr at 34°C. The control was culture Staphylococcus aureus without antimicrobial agents. After that mixture sowed on solid nutrient medium Mueller-Hinton agar for calculation of amount of colony forming particles and MIC definitions. The MIC was considered to be the lowest concentration, which retards the growth of Staphylococcus aureus during the incubation period. 

Reagents and materials Antibiotics, Ampicillin, Mueller Hinton Agar, agar plates were purchased from Meditron Scientific Limited. Bacterial and fimgal cultures were obtained from the Georgetown Public hospital, GPHC. 

In a comparative study of the in vitro antibacterial activity of cefaclor, cephradine, and cephalexin. Silver and co-workers (1977) utilized reported attainable blood levels and mean MICs of each antibiotic to express their potential efficacy as an inhibitory index. The inhibitory index was defined as a ratio between the mean peak serum levels and the mean MIC of isolates susceptible in Mueller-Hinton agar (using an inoculum of 10 organisms/ml). For organisms usually susceptible to cephalosporins (Table X), cefaclor attained the highest inhibitory indexes, demonstrating more antibacterial activity than either cephradine or cephalexin. 

Staphylococcus aureus without antimicrobial agents. After that mixture sowed on solid nutrient medium Mueller-Hinton agar for calculation of amount of colony forming particles and MIC definitions. MIC was considered to be the lowest concentration, which retards the growth of Staphylococcus aureus during the incubation period. 

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