Mucor sp. lipase

Table 11.1-11. Lipase-catalyzed enantiotopos-differentiating hydrolysis of prochiral cyclic diol dialkanoates in aqueous solution (CCL Candida cylindracea lipase, PFL Pseudomonas jiuorescens lipase, MML Mucor miehei lipase, CVL Chromobacterium viscosum lipase, PPL pig pancreas lipase, MJL Mucor javanicus lipase, RSL Rhizopus sp. lipase, PCL Pseudomonas cepacia lipase, CCL, Ceotricum candidum lipase, ANL Aspergillus niger lipase, FSPC Fusarium solani pisi cutinase, CRL Candida rugosa lipase, CAL-B Candida antarctica B lipase, LIP Pseudomonas sp. lipase-Toyobo, RDL Rhizopus delemar lipase, MSL Mucor sp. lipase, CAL Candida antarctica lipase, not specified).

Scheme 2.50 Mirror-image oiicaitation of the eatalytie machinery of Candida rugosa lipase and the protease subtilisin and enantiocomplcanentary ester hydrolysis using Mucor sp. lipase and a-chymotrypsin...

As a rule of thumb, the most widely used lipases may be characterized according to the steric requirements of their preferred substrate esters (Fig. 2.13). Whereas Aspergillus sp. lipases are capable of accepting relatively bulky substrates and therefore exhibit low selectivities on narrow ones, Candida sp. lipases are more versatile in this regard. Both the Pseudomonas and Mucor sp. lipases have been found to be highly selective on substrates with limited steric requirements and hence are often unable to accept bulky compounds. Thus, substrates which are recognized with moderate selectivities by a Candida lipase, are usually more... 

Enzyme activity varies greatly depending on solvent choice, as illustrated by Zaks and Klibanov for the transesterification of tributyrin and heptanol by three different lipases. Using these data, Laane et found that enzyme activity correlates closely with solvent hydrophobicity (log P) for the lipases from Mucor sp. (MML) and Candida cylindracea... 

TIL Thermomyces lanuginosus lipase, RdL Rhizopus delemar lipase, RnL Rhizopus niveus lipase, MmE Mucor miehei esterase, PsL Pseudomonas sp. lipase, MmL Mucor miehei lipase, RoL Rhizopus orvzae lipase, CaLA Candida antarctica lipase A, CaLB Candida antarctica lipase B, PLE Pig liver esterase, EP Enteropeptidase, PKA Porcine kidney acylase, CE Cholesterol esterase Figure 8.1 (S)-Selective enzyme hits from hydrolase screening. ... 

MML Mucor miehei lipase, CAL-B Candida antarctica B lipase, LIP Pseudomonas sp. lipase -Toyobo, HSL Humicola sp. lipase). 

Rhizopus delemar Rhizopus japonicus Alcaligenes sp. Mucor miehei lipases CO2 triolein + stearic acid 290 bar 50 °C [46,47]... 

Lipase from Mucor sp. Synthesis of esters, etc. Enzyme in PVA [28]... 

Lipases from Mucor species (MSL) [9, 473] such as M. miehei and M. javanicus (also denoted as Rhizomucor) have frequently been used for biotransformations [401, 474]. With respect to the steric requirements of substrates they seem to be related to the Pseudomonas sp. lipases. Similar to Candida and Pseudomonas sp. lipases, the different MSL preparations are related in their hydrolytic specificity [39]. 

Candida rugosa lipase (CRL), Aspergillus niger lipase (ANL), Pseudomonas sp. lipase (PSL) and Mucor miehei lipase (MML), have been used for the construction of polyesters from the hydroxyacids and their esters (Table 12.1) in accordance with the following scheme [7] ... 

The ROP of six-membered cyclic carbonate and l,3-dioxan-2-one has been investigated using lipases derived from Candida antarctica, Candida cylmdracea, porcine pancreas. Pseudomonas sp. and Mucor sp. (Scheme 12.10) [47-50]. In some of these reports. 

The biotechnological production of flavour compounds is particularly focused on esters and lactones. Lipase from Mucor miehei is the most widely studied fungal lipase [30-35]. Esters of acids from acetic acid to hexanoic acid and alcohols from methanol to hexanol, geraniol and citronellol have been synthesised using lipases from Mucor miehei, Aspergillus sp., Candida rugosa, Rhizopus arrhizus and Trichosporum fermentans [32-37]. 

Chi et al. (1988) Batch Esterification of triolein using stearic acid Lipases from Rizopus delemar, Rhizopus japonicus, Alcaligenes sp., and Mucor miehei... 

Nakamura et al. (1990) studied the esterification of triolein with stearic acid using four different lipases Mucor miehei, Rhizpous delmar, Alcaligenes sp. and Rhizopus japonicus. They varied the water content of the system... 

Lipase A. oryzae, Aspergillus terreus. Pseudomonas sp., Alcaligenes sp.. Staphylococcus sp., Candida albicans, Rhizopus sp., Mucor... 

A number of points are worth mentioning. All of the reactions described took place at 50-60°C, so isomerization of the CLA was unlikely. Two of the immobilized lipases (those from Mucor miehei and Candida antarctica sp.)... 

---