Mucor javanicus lipase

Balcao, V.M., Malcata, F.X. 1998b. Interesterification and acidolysis of butterfat with oleic acid by Mucor javanicus lipase changes in the pool of fatty acid residues. Enz. Microb. Technol. 22, 511-519. 

Table 11.1-11. Lipase-catalyzed enantiotopos-differentiating hydrolysis of prochiral cyclic diol dialkanoates in aqueous solution (CCL Candida cylindracea lipase, PFL Pseudomonas jiuorescens lipase, MML Mucor miehei lipase, CVL Chromobacterium viscosum lipase, PPL pig pancreas lipase, MJL Mucor javanicus lipase, RSL Rhizopus sp. lipase, PCL Pseudomonas cepacia lipase, CCL, Ceotricum candidum lipase, ANL Aspergillus niger lipase, FSPC Fusarium solani pisi cutinase, CRL Candida rugosa lipase, CAL-B Candida antarctica B lipase, LIP Pseudomonas sp. lipase-Toyobo, RDL Rhizopus delemar lipase, MSL Mucor sp. lipase, CAL Candida antarctica lipase, not specified).

Coupled Mucor javanicus lipase and soybean lipoxygenase... 

H- w-Butyl Mucor javanicus lipase (Fluka)/buffer-saturated isooctane 752 37 38.8 7.7 8.5 1.3 99... 

Mucor javanicus lipase saturated isooctane Mucor javanicus lipase saturated isooctane Mucor javanicus lipase saturated isooctane Mucor javanicus lipase saturated isooctane Mucor javanicus lipase saturated isooctane Mucor javanicus lipase saturated isooctane Mucor javanicus lipase saturated isooctane Mucor javanicus lipase saturated isooctane Mucor javanicus lipase saturated isooctane Mucor javanicus lipase saturated isooctane... 

Nagayama, K. and Imai, M. (2005) Enhanced activity of Mucor javanicus lipase in polyoxyethylene sorbitan trioleate containing microemulsion-based organogels. J. Mol. Catal. B Enzym.,... 

Lipase M (Mucor javanicus), pH 7, 37°, 70-88% yield. In this case, a- and )3-glycosidic peptide derivatives were deprotected. Acetates on the pyrano-sides were not affected. ... 

In our previous work [63], we studied the hydrolysis kinetics of lipase from Mucor javanicus in a modified Lewis cell (Fig. 4). Initial hydrolysis reaction rates (uri) were measured in the presence of lipase in the aqueous phase (borate buffer). Initial substrate (trilinolein) concentration (TLj) in the organic phase (octane) was between 0.05 and 8 mM. The presence of the interface with octane enhances hydrolysis [37]. Lineweaver-Burk plots of the kinetics curve (1/Uj.] = f( /TL)) gave straight lines, demonstrating that the hydrolysis reaction shows the expected kinetic behavior (Michaelis-Menten). Excess substrate results in reaction inhibition. Apparent parameters of the Michaelis equation were determined from the curve l/urj = f /TL) and substrate inhibition was determined from the curve 1/Uj.] =f(TL) ... 

Free lipase from Mucor javanicus Octane-borate buffer pH 9.6 (1/1) Trilinolein hydrolysis 25°C 9.8 /xM/min 192 pM Kss = 5300 /xM 63... 

The full capacity of the lipase-mediated technique for C-terminal deprotection is demonstrated in the synthesis of complex C>-glycopeptides, which are sensitive to both acids and bases, The key starting material, serine glycoside heptyl ester 11, is selectively depro-tected at the C-terminus by lipase obtained from the fungus Mucor javanicus (Scheme 22). The free carboxylic acid derivative 12 is coupled with an N-terminally protected glyco-dipeptide 13 to generate the tripeptide 14. 

Scheme 23 Lability and Stability of the C-Terminal (2-Methoxyethoxy)ethyl Ester of Glycopeptides to Lipases from Mucor javanicus and Wheat Germl ...

In addition to these examples, the lipase from Mucor javanicus accepts various further glycosylated amino acids and peptides as substrates and selectively deprotects them at the C-terminus (Fig. 13). The enzyme tolerates varia-... 

Interesterification can also be catalysed by enzymes, many of which show useful specificities. The 1,3-specific lipases, such as those derived from Aspergillus niger, Mucor javanicus, M. miehei, Rhizopus arrhizus, R. delemar, and R. niveus, are particularly useful for interesterification. They are used to effect acyl exchange at the sn- and 3 positions while leaving acyl groups at the sn-2 position unchanged. 

Lipase Origin Mucor javanicus Amano Lipase M "Amano" 10... 

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