Amano lipase

Lipase Amano PS-C II was also found to be useful for a high-temperature reaction in the resolution of a bulky substrate, l,l-diphenyl-2-propanol, which showed no reactivity under usual conditions with lipase PS. An enantiopure product was obtained at 40-120°C, and the highest conversion (39%) was obtained at 80-90°C. It is very interesting that a single enzyme is usable in the reaction at a very wide range of temperatures from —80°C to 120°C. 

Burkholderia cepacia lipase (Amano PS) 2,2 -Bis(diphenylphosphino)-1,1 -binaphthyl Candida antarctica lipase B Diversity-oriented synthesis... 

A number of racemic 2-aryloxy-1-propanols 8 have been resolved by Pseudomonas sp. lipase (Amano AK)-catalyzed acylation in diisopropyl ether (DIPE) at 25 °C (Scheme 4.9) [46]. Because an oxygen atom is directly attached to the stereogenic center, the selection rule described in Figure 4.3 does not hold. 

Lipase Amano AK, vinyl acetate, benzene 37 °C, 5 days, 68%, 95% . 

Optically active aziridines have been prepared in high enantiomeric excess by the enzymatic resolution of meso diesters (94AG(E)599). For example, when the me o-bis(acetoxymethyl)aziridine (56) was subjected to enzymatic hydrolysis with lipase Amano P, the aziridine (57) was obtained in 98% ee (90TL6663). 

Residual activity of different enzymes after 1 hour treatment in supercritical CO2 at 150 bar (I, lipase from Candida cylindracea If, lipase Amano AY III, lipase from Pseudomonas sp. IV, esterase EP10 from Burkholderia gladioli) ... 

Theil, F., Femke, K., Ballschuh, S., Kunath, A., and Schick, H. 1995. Fipase-catalyzed resolution of 3-(aryloxy)-l,2-propanediol derivatives-towards an improved active-site model of Pseudomonas cepacia lipase (Amano PS). Tetrahedron Asymm., 6, 1323-1344. 

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