MRNA turnover

Although we will stick to the IL-6 gene, it should be mentioned at the side that two other RNA polymerases exist in mammalian cells responsible for the synthesis of RNA molecules, which are not translated into proteins ribosomal (rRNA), transfer (tRNA), small nuclear (snRNA), small nucleolar (snoRNA), and some of the recently discovered microRNAs and piRNAs. These RNA molecules act in the process of translation and mRNA turnover. Micro and piRNAs are probably extremely important in the definition of stem cells and of differentiation programs. Some of them are synthesized by RNA polymerase II. 

Beelman, C. A., Stevens, A., Caponigro, G., LaGrandeur, T. E., Hatfield, L., Fortner, D. M., and Parker, R. (1998). An essential component of the decapping enzyme required for normal rates of mRNA turnover. Nature 382, 642-646. 

Leeds P, Wood JM, Lee BS, Culbertson MR (1992) Gene products that promote mRNA turnover in Saccha-... 

Ruiz-Echevarria Ml, Yasenchak JM, Han X, Dinman JD, Peltz SW (1998) The upf3 protein is a component of the surveillance complex that monitors both translation and mRNA turnover and affects viral propagation. Proc Natl Acad Sci USA 95 8721—8726... 

Weng Y, Czaplinski K, Peltz SW (1996) Identification and characterization of mutations in the UPFl gene that affect nonsense suppression and the formation of the Upf protein complex but not mRNA turnover. Mol Cell Biol 16 5491-5506... 

Zuk D, Belk JP, Jacobson A (1999) Temperature-sensitive mutations in the Sacchammyces cerevisiae MRT4, GRC5, SLA2 and THS1 genes result in defects in mRNA turnover. Genetics 153 35-47... 

Jacobs, J. S., Anderson, A. R., and Parker, R. P. (1998) The 3 to 5 degradation of yeast mRNAs is a general mechanism for mRNA turnover that requires the SKI2 DEVH box protein and 3 to 5 exonucleases of the exosome complex. EMBO J. 17, 1497-1506. 

PeltzSW, Brown AH, Jacobson A Regulation of mRNA turnover in eukaryotic cells. Crit Rev Eukaiyot Gene Exp ession 1 99-126,1993. 

Hagan KW, Ruiz-Echevarria MJ, Quan Y, Peltz SW Charaderization of d -acting sequences and decay intermediates involved in nonsense-mediated mRNA turnover. Mol Cell Biol 15 809-823,1995. 

Lam LT, Pickeral OK, Peng AC, Rosen-wald A, Hurt EM, et al. 2001. Genomic-scale measurement of mRNA turnover and the mechanisms of action of the anticancer drug flavopiridol. Genome Biol. 2 41.1—41.11... 

See also mRNA Turnover, Ribozymes (from Chapter 11), Structure of tRNAs (from Chapter 27), Structure of Prokaryotic mRNAs (from Chapter 27)... 

Blackshear P J (2002). Tristetraprolin and other CCCH tandem zinc-finger proteins in the regulation of mRNA turnover. Biochem. Soc. Trans. 30 945-952. 

Malissen So your hypothesis would be that Roquin has something to do with mRNA turnover ... 

Tebbey, PW., et al., Arachidonic acid down-regulates the insulin-dependent glucose transporter gene (Glut 4) in 3T3-L1 adipocytes by inhibiting transcription and enhancing mRNA turnover, J. Biol. Chem., 269, 639, 1994. 

Larsson, E., Sander, C., Marks, D. (2010). mRNA turnover rate limits siRNA and microRNA efficacy. Mol Syst Biol, 16 (6), 433. 

Chaperonins Protein folding Oligomer assembly Protein transport DNA replication mRNA turnover 2,3... 

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