Moving boundary analysis

Martin and Everaerts published a paper in 1967 which gave a new impulse. Their capillary equipment for displacement electrophoresis had already the character of the present devices. They used as a detector a thermocouple glued on the outside wall of a thin-walled separation capillary. The principle of and the equipment for the moving boundary analysis with a detection performed potentio-metrically was reported by Hello in 1968. One year later, Fredriksson developed the analytical apparatus for the displacement electrophoresis . He used a conductivity detector, successfully separated low-molecular fatty acids and performed quantitative an dyses by measuring the lengths of zones. 

The analysis of fluid-solid reactions is easier when the particle geometry is independent of the extent of reaction. Table 11.6 lists some situations where this assumption is reasonable. However, even when the reaction geometry is fixed, moving boundary problems and sharp reaction fronts are the general rule for fluid-solid reactions. The next few examples explore this point. 

The origins, principles, methods, and modes of capillary electrophoresis (CE) are discussed. Massive application of electrophoresis methods started after Tiselius s moving boundary method that was optimized by the use of paper or a gel as a semiconducting medium. The applications of paper and gel electrophoresis were situated mostly in the biochemical environment for the analysis of proteins, amino... 

Also known as copper blue. maunt on blu ) moving-boundary electrophoresis analychem A U-tube variation of electrophoresis analysis that uses buffered solution so that all ions of a given species move at the same rate to maintain a sharp, moving front (boundary). miivii) baun-dre i lek-tro-fo re-sos ... 

AktivKemiMineralGeol A22, No 10(1946) (Electrophoresis by the moving boundary method) 5) A. Weissberger, Physicochemical Methods of Organic Analysis , VanNostrand, NY, Vol 1(1949), pp 1685—1712 6) R.E. Kirk D.F. Othmer, Edits, Encyclopedia of Chemical Technology", Interscience,... 

Let us briefly outline the main concepts of a (linear) stability analysis and refer to the situation illustrated in Figure 11-7. If we artificially keep the moving boundary morphologically stable, we can immediately calculate the steady state vacancy flux, /v, across the crystal. The boundary velocity relative to the laboratory reference system (crystal lattice) is... 

Differential ultracentrifugation methods may also be applied to analysis of the purity of macromolecular samples. If one sharp moving boundary is observed in a rotating centrifuge cell, it indicates that the sample has one component and therefore is pure. In an impure sample, each com ponent would be expected to form a separate moving boundary upon sedimentation. 

From the analysis of the kinematic conditions at a moving boundary 36), with Eq. (4.26) taken into account, we obtain ... 

E. X. Vrouwe, R. Luttge, W. Olthuis and A. van den Berg, Microchip analysis of lithium in blood using moving boundary electrophoresis and zone electrophoresis, Electrophoresis, 26 (2005) 3032-3042. 

Aug. 10,1902, Stockholm, Sweden - Oct. 29,1971, Uppsala, Sweden) Tiselius studied chemistry in Uppsala and joined the laboratory of -> Svedberg in 1925. In 1930 he received the Ph.D. for his thesis entitled The moving-boundary method of studying the electrophoresis of proteins . In 1937, a research professorship was established for Tiselius. His fundamental work in developing -> electrophoresis was encouraged and supported by Svedberg. In 1948 he was awarded the Nobel Prize in Chemistry for his achievements in electrophoresis and adsorption analysis. 

Electrophoresis experiments in glass tubes were reported as early as in the nineteenth century, but the first real breakthrough occurred in the first half of the twentieth century when the Swedish chemist Arne Tiselius applied free-solution electrophoresis—i.e moving boundary—to serum protein analysis, for which he later received the 1937 Nobel Prize [2], In less than two decades, just after the striking scientific discovery of the double-helical structure of DNA by Watson and Crick in 1953 [3] and the following unveiling of the genetic code, electrophoresis became a standard and indispensable tool in the field of modern... 

Transference Numbers in Mixtures.—Relatively little work has been done on the transference numbers of ions in mixtures, although both Hittorf and moving boundary methods have been employed. In the former case, it follows from equation (3) that the transference number of any ion in a mixture is equal to the number of equivalents of that ion migrating from the appropriate compartment divided by the total number of equivalents deposited in a coulometer. It is possible, therefore, to derive the required transference numbers by analysis of the anode and cathode compartments before and after electrolysis. 

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