Mouse sperm tyrosine phosphorylation

In attempts to further understand the signal transduction cascades that regulate capacitation, our laboratory has recently correlated mouse, human, and bovine sperm capacitation with an increase in protein tyrosine phosphorylation of a variety of substrates (Visconti et ah, 1995a Carrera et ah, 1996 Galantino-Homer et ah, 1997). Many of these results have since been corroborated by other labs (Aitken et ah, 1995 Leclerc et ah, 1996 Luconi et ah, 1996 Emiliozzi and Fenichel, 1997). Using the mouse as an experimental paradigm, our laboratory has demonstrated... 

Visconti, P.E., Moore, G.D., Bailey, J.L., Leclerc, P., Connors, S.A., Pan, D., Olds-Clarke, P., and Kopf, G.S. (1995b). Capacitation of mouse spermatozoa. 11. Protein tyrosine phosphorylation and capacitation are regulated by a cAMP-dependent pathway. Development I21 1139-1150. Visconti, P.E., Johnson, L., Oyaski, M., Pomes, M., Moss, S.B., Gerton, G.L., and Kopf. G.S. (1997). Regulation, localization, and anchoring of protein kinase A subunits during mouse sperm capacitation. Dev. Biol. 792 351-363. 

Tomes, C.N., McMaster, C.R., and Saling, P.M. (1996). Activation of mouse sperm phosphatidylinositol-4,5 bisphosphate-phospholipase C by zona pellucida is modulated by tyrosine phosphorylation. Mol. Reprod. Dev. 45 196-204. 

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