Mouse sperm capacitation

Visconti, P.E., Moore, G.D., Bailey, J.L., Leclerc, P., Connors, S.A., Pan, D., Olds-Clarke, P., and Kopf, G.S. (1995b). Capacitation of mouse spermatozoa. 11. Protein tyrosine phosphorylation and capacitation are regulated by a cAMP-dependent pathway. Development I21 1139-1150. Visconti, P.E., Johnson, L., Oyaski, M., Pomes, M., Moss, S.B., Gerton, G.L., and Kopf. G.S. (1997). Regulation, localization, and anchoring of protein kinase A subunits during mouse sperm capacitation. Dev. Biol. 792 351-363. 

Percoll gradient-centrifuged capacitated mouse sperm have in- 118. creased fertilizing ability and higher contents of sulfogalactosyl-glycerolipid and docosahexaenoic acid-containing phosphatidylcholine compared to washed capacitated mouse sperm. Biol. 119. Reprod. 2005 72 574-583. 

In attempts to further understand the signal transduction cascades that regulate capacitation, our laboratory has recently correlated mouse, human, and bovine sperm capacitation with an increase in protein tyrosine phosphorylation of a variety of substrates (Visconti et ah, 1995a Carrera et ah, 1996 Galantino-Homer et ah, 1997). Many of these results have since been corroborated by other labs (Aitken et ah, 1995 Leclerc et ah, 1996 Luconi et ah, 1996 Emiliozzi and Fenichel, 1997). Using the mouse as an experimental paradigm, our laboratory has demonstrated... 

Zeng, Y, Oberdorf J.A., and Florman, H.M. (1996). pH regulation in mouse sperm. Identification of Na, Cl, and HCOj -dependent and arylaminobenzoate-dependent regulatory rhechanisms and characterization of their role in sperm capacitation. Dev. Biol. 773 510-520. 

Duncan, A.E. and Fraser, L.R. (1993). Cyclic AMP-dependent phosphorylation of epididymal mouse sperm proteins during capacitation in vitro Identification of an M, 95 000 phosphotyrosine-containing protein. J. Reprod. Fertil. 97 287-299. 

Wolf, D.E., Hagopian, S.S., and Isogima, S. (1986). Changes in sperm plasma membrane lipid diffusibility after hyperactivation during in vitro capacitation in the mouse. J. Cell Biol. 

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