Monosaccharides spectral study

The most significant feature in the mass spectral studies of cyclic monosaccharide acetates, is the differentiation between aldoses and ketoses and also pyranoid and furanoid ring forms. 

The fraction of protein, eluted from Sephadex G-75 which behaves as a single homogeneous protein with respect to its interactions with M2 was also studied with regard to its interaction with monosaccharides. The association constant of the interaction of M2 with intact con A is 19 times greater than that obtained for the interaction of p-nitrophenyl a-D-mannopyranoside with con A. The normalized spectra (Figure 7) of the monosaccharide and disaccharide complexes were found to be very similar suggesting that the interactions between con A and the nitrophenyl group which causes the spectral perturbation are the same for both complexes. 

Tables I-III give some selected FAB data for anomeric pairs of monosaccharide glycosides in the positive ion mode. Differences occur in the mass spectral data recorded within a given anomeric pair and this is true for every pair studied.

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