Monomethoxy polyethylene glycol

PEG-Intron PEG-Intron is used for the treatment of hepatitis C. The product consists of a covalent conjugate of the recombinant interferon- -2b with monomethoxy polyethylene glycol (PEG) supplied in vials with 74/rg, 118.4/rg, 177.6/rg, or 222/rg of the active ingredient and 1.11 mg sodium phosphate (dibasic,anhydrous),l.ll mg sodium phosphate (monobasic, dihydrate), 59.2 mg sucrose, and 0.074mg polysorbate 80. The powder is reconstituted with sterile water-for-injection. 

Natural interferons produced by human leukocytes, recombinant interferons produced in bacteria, and recombinant interferons conjugated to monomethoxy polyethylene glycol (PEG pegylated interferons) are available in the United States. The various preparations may be administered subcutaneously, intramuscularly, intravenously, or intralesionally (e.g., into genital warts). Natural or recombinant interferons typically achieve peak plasma levels within 4 to 8 hours of subcutaneous... 

Veronese, F.M., Largajolli, R., Boccu, E., Benassi, C.A., and Schiavon, O. (1985). Surface modification of proteins activation of monomethoxy-polyethylene glycols by phe-nylchloroformates and modification of ribonuclease and superoxide dismutase. Alied Biochem. Biotechnol., 11, 141-152. 

Adagen (pegademase bovine) [monomethoxy polyethylene glycol- Enzon Liquid pharmaceuti- (single use vial) cals 250 units/mL (pH 7.2-7.4) Isotonic solution IM... 

Most of these polymers have multi-functional character, which results in cross-linked heterogeneous products. In contrast, monomethoxy polyethylene glycol (PEG) presents only one reactive terminal group per polymer chain. Once PEGy-lated with these compounds, the protein acquires a brush-like shape, with the hydrophilic PEG chains extended from the protein to the solvent. 

Covalent conjugate of recombinant alfa-2a interferon with monomethoxy polyethylene glycol (PEG) (type 1 interferon)... 

Monomethoxy polyethylene glycol succinimidyl succinate (PEG-OSu), mol wt approx 5000, octyl glucoside (OG), cholesterol, stearyl amine (S A), and buffers, such as HEPES and MES, are available from Sigma (St. Louis, MO). 

Richter A W, Akerblom E (1983). Antibodies against polyethylene glycol prodnced in animals by immunization with monomethoxy polyethylene glycol modified proteins. Int. Arch. Allergy Appl. Immunol. 70 124-131. 

Protein modification with polymeric reagents is undertaken for a variety of reasons. Some of these are increased solubility, increased half-life in vivo, modified bioactivity, and reduced antigenicity. We will describe the development and use of protein modifying reagents based on polyproline [H(PRO)x-OH], and monomethoxy-polyethylene glycol (mono-methylpolyoxyethylene, PEG), a molecule with basic formula CH3-0- CH2-CH2-0-)n-H.5 Both of these reagents were chosen for their water solubility and well-defined chemical functionality. 

The second protein modifying group of reagents we sought are based on monomethoxy-polyethylene glycol (M-PEG). Polyethylene glycol has been attached to proteins by using sy/n-trichlorocyanuric acid and by conver-... 

L. Sartore, P. Cahceti, O. Schiavon, C. Monfardini, EM. Veronese, Accurate evaluation method of the polymer content in monomethoxy(polyethylene glycol) modified proteins based on amino acid analysis. Appl Biochem. BiotechnoL, 31 (3) 213-222,1991. 

J. Rostin, A.-L. Smeds, E.B. Akerblom, Domain deleted recombinant coagulation factor VIII modified with monomethoxy polyethylene glycol. Bioconjugate Chem., 11 (3) 387-396, 2000. 

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