Monoclonal antibodies storage

Ciocca DR, Adams DJ, Bjercke RJ, Sledge GW, Edwards DP, Chamness GC, Mcguire WL (1983) Monoclonal antibody storage conditions, and concentration effects on immunohistochemical specificity. J Histochem Cytochem 31 691 696... 

Storage and preparation of polyclonal and monoclonal antibodies are very important for successful immunostaining. When a new antibody arrives in a laboratory, special attention should be paid to its storage instructions and optimal temperature. Detailed records of arrival and expiry dates, and whether it needs to be aliquoted to maintain efficacy by avoiding ffeeze-thaw effects, should be kept. Attention to these details will preserve the efficacy of the antibody and its shelf hfe, prevent damaging freeze-thaw cycles, and will make optimization of the immunohistochemical procedures easier, ft is often necessary to aliquot antibodies, especially when storage below 4°C is required. 

In sum, EMPs have emerged as a preferred direct method for assessing EC injury in different disorders. EMP analysis could provide insight into the actual status of the endothelium in vivo by a simple blood analysis. However, there is a need for refinement and standardization of the assay method. Overall, most groups have relied on flow cytometry for the measurement of EMPs nevertheless, other methods such as ELISA are available and may be an option in the future. The main challenge remains in the selection of specific and sensitive monoclonal antibodies that may yield consistent results between different laboratories. In addition, the protocols for sample handling and storage need to be clearly delineated. The assay is still a... 

Monoclonal antibodies also have been shown to be influenced in their performance by methods of purification and storage 42 percent of monoclonal antibodies investigated by Underwood and Bean showed changes in specificity, affinity and cross-reactivity (14). Antibodies of class IgM and subclass lgG2b were especially sensitive. 

Mouse-anti-/3-galactosidase monoclonal antibody (Jl) —Keep the stock antibody (Sigma catalog G-8021) frozen at — 80°C. We have found that the titer drops dramatically after short-term storage at — 20°C. Dilute the antibody immediately before use in blocking solution (about a 1 1000 dilution). Each group will require about 1.2 ml of the diluted antibody to perform the three-day experiment. 

Figure 10 The stability of a freeze-dried monoclonal antibody/vinca alkaloid conjugate formulation. Desacetylvinblastine hydrazide is linked to the KSl/4 monoclonal antibody via aldehyde residues of the oxidized carbohydrate groups on the antibody. The formulation is conjugate/glycine/mannitol in a I I I weight ratio. Storage temperatures are 25°C and 40°C for samples with moisture contents of 1.4%, 3.0%, and 4.7%. ( ) Dimer formation. (A) Free vinca generation (hydrolysis). ( ) Vinca degradation. (—) Best fit to the WLF equation. (Reproduced with permission from [54].)...

Cleland JL, Lam X, Kendrick B, Yang J, Yang T, Overcashier D, Brooks D, Hsu C, Carpenter J (2001) A specific molar ratio of stabilizer to protein is required for storage stability of a lyophilized monoclonal antibody. J Pharm Sci 90(3) 310-321. 

In the beverage industry it is used to improve product yield and quaUty. UF systems remove proteins, suspended colloids, polyphenoHc compounds, starch, pectin, and microorganisms from natural juice providing a brilliantly clear juice (permeate) that is stable even after extended storage. UF is commonly used for product concentration (i.e., solvent removal) in bioprocessing concentration of cell-free fermentation broths containing complex biological compounds such as monoclonal antibodies as well as biomolecule/product recovery from very dilute solutions. UF systems are also used for virus removal in the production of therapeutic proteins and vaccines and for antibiotics recovery. 

Memikweu, P., Majumdar, R., Hickey, J.M., et al. (2013) Correlating excipient effects on conformational and storage stabihty of an IgGl monocloned antibody with local dynamics as measured by hydrogen/ deuterium-exchange mass spectrometry. J Pharm Sci, 102 (7), 2136-2151. 

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