Monoclonal antibodies anti-platelet

Monoclonal antibodies Anti-platelets, anti-CD52, a-cancer M195... 

Ellis, S. G., Tcheng, J. E., Navetta, F. I., Muller, D. W. M., Weisman, H. F., Smith, C., Anderson, K. M., Califf, R. M., and Topol, E. J. (1993). Safety and anti-platelet effect of murine monoclonal antibody 7E3 Fab directed against platelet glycoprotein Ilb/IIIa in patients undergoing elective coronary angioplasty. Coronary Artery Dis. 4, 167-175. 

Monoclonal antibodies are coming onto the market at an increasing rate. For example, abciximab is an anti-platelet drug, trastuzumab is active against breast cancer, and adali-mumab and inflixamab treat rheumatoid arthritis. The suffix mab is a convenient guide to products in this category. 

Meisenhelder, J., and Hunter, T. (1992) The SH2/SH3 domain-containing protein Nek is recognized by certain anti-phospholipase C-yl monoclonal antibodies and its phosphorylation on tyrosine is stimulated by platelet-derived growth factor and epidermal growth factor treatment. Mol. Cell. Biol. 12, 5843-5856. 

Aliquots of Sepharose 4B (0.5 ml settled volume) or Sepharose 4B coupled to anti-cathepsin M, or to a monoclonal antibody directed against human platelet glycoprotein, as indicated, were washed with 0.25 M sucrose containing 10 mM sodium borate, pH 7.4. The resins were then suspended in 2 ml of the same buflFered sucrose solution containing 1 mg/ml of bovine serum albumin and 100/tl of the heavy particle fraction (0.99 mg/ml of protein). The mixtures were rotated end-over-end for 30 minutes at 5°C and the resins collected by free sedimentation and washed six times with the same buffered isotonic sucrose mixture. The washed gels were suspended in 0.5 ml of 0.5% Triton X-100 and after 10 minutes at room temperature the supernatant solutions were assayed for cathepsin C activity. From Pontre-moli et al. (31) reproduced with permission. 

Also in support of the location of cathepsin M on the outer lysosomal surface were results obtained with a monoclonal antibody prepared against the rabbit liver enzyme. This antibody, but not another monoclonal antibody specific for platelet surface markers, could be shown to bind to the intact lysosomes. In addition, rabbit liver lysosomes could be shown to bind to monoclonal anti-cathepsin M coupled to Sepharose 4B, but not to Sepharose 4B alone or to a coupled monoclonal antibody directed against surface glycoprotein of human platelets (Table II). 

Matsuno K, Diaz-Ricart M, Montgomery RR, Aster RH, Jamieson GA, Tandon NN Inhibition of platelet adhesion to collagen by monoclonal anti-CD36 antibodies. Br J Haematol 92 960-967,1996... 

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