Monoclonal antibodies activity loss

Kobayashi, H., Sato, N., Saga, T., Nakamoto, Y., Ishimori, T., Toyama, S., Togashi, K., Konishi, J., and Brechbiel, M.W. (2000) Monoclonal antibody-dendrimer conjugates enable radiolabeling of antibody with markedly high specific activity with minimal loss of immunoreactivity. Eur. J. Nucl. Med. 27, 1334-1339. 

It has been demonstrated that monoclonal antibodies may be chemically modified by reaction with metal chelates without loss of antibody activity or specificity. A radionuclide generator has been made to provide a source for Bi-212 to be used for attachment of bismuth chelates to antibody. Such antibody-metal-chelate conjugates appear to be stable in vivo and may provide a new method for radiotherapy. 

Immobilized monoclonal antibodies represent yet another useful matrix for reversible immobilization. Because these antibodies are monospecific and can be chosen so as to correspond to epitopes on enzyme surface and not directed against enzyme active site, the immobilization does not result in significant loss of activity. Solomon et al. (45) describes immobilization of carbodypep-tidase A. 

Can perturbations to the rod affect the activity of a phosphorylated myosin head, which would normally be active A monoclonal antibody that binds very near to the C terminus of the myosin rod and depolymer-izes myosin filaments caused a loss in motility and actin-activated ATPase activity (Horowitz and Trybus,... 

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