Molecularly imprinted SPE MISPE

SAMPLE APPLICATION, WASHING AND ELUTION CONDITIONS IN PREVIOUS MISPE PROTOCOLS. For structures of the analytes see page 361 

A Pentamidine 1 Column HPLC Off-line Urine Dilution with buffer MeCN/buffer pH 9 MeCN/ uv [18] 

B 7-Hydroxy- Cartridge — Off-line Urine pH adjustment (6.0) Water MeOH CZE [26] 

C Sameridine 3 Batch Radioligand Off-line Plasma Extraction in heptane Heptane EtOH Heptane/ GC-FID [23] 

D Siinazine 4 Cartridge HPLC Off-line Water None Dichloromethane MeOH HPLC-UV [19] 

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Molecularly imprinted SPE (MISPE) is a type of SPE that is more time-consuming than simple SPE since the molecularly imprinted polymer (MIP) stationary phase has to be specifically synthesized for the target dye recognition. Recently a titania-based MIP was synthesized through a sol-gel process using Sunset Yellow as template, without use of a functional monomer [27]. This MIP was used as an SPE material for the isolation and enrichment of sulfonic acid dyes in beverages. The better cleanup ability demonstrated the capability of MIP for the isolation and enrichment of sulfonic acid dyes in complicated food samples. 

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