Molecularly imprinted polymers, preparation

Molecularly Imprinted Polymers -Preparation, Biomedical Applications and Technical Challenges... 

Rachkov, A., Hu, M., Bulgarevich, E., Matsumoto, T., and Minoura, N. (2004) Molecularly imprinted polymers prepared in aqueous solution selective for [Sar Ala8] angiotensin II. Analytica Chimica Acta, 504,191 197. 

Molecularly imprinted polymers -preparation, biomedical applications and technical challenges Allender, C. J. Brain, K. R. Heard, C. M. Prog. Med. Chem. 1999, 36, 235-291. 

Torres, J. J., Gsponer, N., Ramirez, C. L., Vera, D. M., Montejano, H. A., Chesta, C. A. (2012). Experimental and theoretical studies on the enantioselectivity of molecularly imprinted polymers prepared with a chiral functional monomer. I Chromatoar. A. 1266,24-33. 

Scheme 13 Schematic representation of molecularly imprinted polymer preparation for P-17 and subsequent use to catalyze the aldol condensation between acetophenone, 29, and benzaldehyde, 30.

The use of surfactants that are also able to bind molecules of interest has long been of interest in the preparation of molecularly imprinted polymers, prepared by emulsion polymerization processes [12-21]. Surfactants containing functionality capable of binding to metal ions have been used in emulsion polymerization. The bifunctional surfactant l,12-dodecane-diol-0,0 -diphenyl phosphonic acid (Fig. 3) was used in the synthesis of polymers imprinted with zinc ions in a water in oil emulsion [22]. This a,co-functional surfactant sits at the interface between the organic and aqueous phases during polymerization. 

Masci, G. Aulenta, F. Crescenzi, V. Uniform-sized clembuterol molecularly imprinted polymers prepared with methacrylic acid or acrylamide as an interacting monomer. J. Appl. Polym. Sci. 2002, 83, 2660-2668. 

Haginaka, J. Takehira, H. Hosoya, K. Tanaka, N. Molecularly imprinted uniformsized polymer-based stationary phase for naproxen. Comparison of molecular recognition ability of the molecularly imprinted polymers prepared by thermal and redox polymerization techniques. J. Chromatogr. A 1998, 816, 113-121. 

Andersson, L.I. Mosbach, K. Enantiomeric resolution on molecularly imprinted polymers prepared with only non-covalent and non-ionic interactions. J. Chromatogr. 1990, 516, 313-322. 

Song, X., J. Li, J. Wang, and L. Chen. 2009. Quercetin molecularly imprinted polymers Preparation, recognition characteristics and properties as sorbent for solid-phase extraction. Talanta 80(2) 694-702. 

Fig. 5-5. Schematic representation of the preparation procedure of molecular imprinted polymers (MIP).

Molecularly imprinted polymers (MIPs) can be prepared according to a number of approaches that are different in the way the template is linked to the functional monomer and subsequently to the polymeric binding sites (Fig. 6-1). Thus, the template can be linked and subsequently recognized by virtually any combination of cleavable covalent bonds, metal ion co-ordination or noncovalent bonds. The first example of molecular imprinting of organic network polymers introduced by Wulff was based on a covalent attachment strategy i.e. covalent monomer-template, covalent polymer-template [12]. 

Molecularly imprinted polymers have recently attracted much attention because they are denoted as artificial antibodies which are made from simple chemical components via polymerization and can be used for the preparation ofbiomimetic sensors, affinity separation matrices, catalysts, etc. (Figure 1). 

Figure 2. Preparation of coloured molecularly imprinted polymers using analyte sensitive indicator dyes.

Molecularly imprinted polymers (MIPs) are of growing interest for their potential biotechnological applications. Recently, the templating processes with living yeast cells were reported107 for the preparation of ordered and... 

This section will illustrate the MIP technique for sample preparation by presenting examples of diazepam and its metabolites in hair samples.177 An anti-diazepam molecularly imprinted polymer... 

FIGURE 13.12 Preparation of a molecularly imprinted polymer. (Reproduced from Kim, H.S. and Hage, D.S., Immobilization methods for affinity chromatography, in Handbook of Affinity Chromatography, 2nd edn., Hage, D.S. (ed.), CRC Press, Boca Raton, FL, 2005, Chap. 3.)... 

Figure 15.1 Examples of common cioss-liiikers used in the preparation of molecular imprinted polymers ethylene glycol dimethylcrylate (EGDMA) divinyl benzene (DVB), trimethylolpropane trimethacrylate (TRIM), VA -methylenebisacrylamide (MBA), and V,0-bismethacryloyl ethanolamine (NOBE).

Schweitz L. Molecularly imprinted polymer coatings for open-tubular capillary electrochromatography prepared by surface initiation. Anal Chem 2002 74 1192-1196. 

Fig. 12 (a) Schematic representation of the preparation of a molecularly imprinted polymer with immobilized Au nanoparticle and the detection of an analyte upon selective swelling of the MIP. Reprinted with permission from [123]. Copyright (2004) American Chemical Society, (b) Schematic representation of Au-MIP/MIP-coated SPR sensor chip. Reprinted with permission from [124]. Copyright (2005) American Chemical Society... 

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