Molecularly imprinted polymers binding capacity

Chianella, I. Karim, K. Piletska, E. V. Preston, C. Piletsky, S. A., Computational design and synthesis of molecularly imprinted polymers with high binding capacity for pharmaceutical applications-model case Adsorbent for abacavir, Anal. Chim. Acta. 2006, 559, 73-78... 

This system can be used on and off line. These cartridges have a high capacity for binding of small molecules and contain different bonding phases, ranging from silica gel, C-18 (octadecylsilane), florisil, phenyl, aminopropyl, ion exchange materials (both anionic and cationic), to affinity materials such as immunoadsor-bents and molecular imprinted polymers (MIPs) [38-49]. 

CNTs were used successfully to enhance the binding capacity of a molecularly imprinted polypyrrole modified stainless steel frit for determination of ochratoxin A in red wines (Yu J.C.C. Lai, 2007 Wei et al., 2007). In a different work (li Y. et al., 2010), a molecularly imprinted polymer-graphene oxide hybrid material was synthesized by reversible addition and fragmentation chain transfer (RAFT) pwlymerization for the selective detection of 2,4-dichlorophenol in aqueous solution with an ajipredable selectivity over structurally related compounds. 

The first example, depicted in Fig. 6, describes the synthesis and evaluation of a polymer imprinted with the bronchodilating drug theophylline, which is used in the treatment of asthma. Originally published in the journal Nature [2], this work drew considerable attention to the field of molecular imprinting because it was the first study to show that an MIP could be substituted for a natural antibody in a standard clinical assay. The MIP and antibody-based assays exhibit similar selectivities, and both can discriminate between theophylline and structurally related compounds. An equilibrium binding assay is described which uses radiolabeled theophylline as a marker. Data are presented for which nonradioactive theophylline, caffeine, and theobromine are used in competitive binding assays. These assays provide valuable information about the capacity and selectivity of the MIP. 

Sellergren, B. Molecular imprinting by noncovalent interactions. Enantioselectivity and binding capacity of polymers prepared under conditions favoring the formation of template complexes. Makromol. Chem. 1989,190, 2703-2711. 

Sellergren, B. Molecular imprinting by noncovalent interactions—enantioselectivity and binding-capacity of polymers prepared tmder conditions favoring the formation of template complexes. Makromolek. Chem.—Macromol. Chem. Phys. 1989, 190, 2703-2711. Kempe, M. Mosbach, K. Directed resolution of naproxen on a non-covalently molecularly imprinted chiral stationary phase. J. Chromatogr. 1994, 664, 276-279. 

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