Molecular imprinting base pairing

Acid/base chemistry is also an obvious reaction path for selective detection of a variety of materials. For example, acid/base reaction can be made to occur within a molecularly imprinted pocket to assist in differentiating the molecule of choice before deprotonating it to produce an ion pair that then interacts with the evanescent field. This approach is currently being used to selectively detect TNT to levels in the low parts-per-trillion. 2,4,6-trinitrotoluene, a potent explosive, is also a weak acid having a p/sTa of approximately 14.5 [38]. A TNT derivative is synthesized with a tether to a silane. The silane is chosen so the group can be... 

In the context of molecular imprinting, ionic interactions occur as a result of a proton transfer in a Bronsted acid/base type process. Alternatively, this type of ionic interaction can be viewed as an extension of the hydrogen bonding process. Figure 6.22 illustrates that the proton transfer ion-pair is in equilibrium with the covalent H-bonded complex, the position of the equilibrium being dependent upon the pKas of the acidic and basic groups and the solvent environment [34]. 

Many planar dye molecules are bound to double-stranded DNA by being accommodated between adjacent Watson-Crick base pairs. These non-covalent interactions (intercalation) are used for molecular imprinting [11], Various hydrophobic interactions are also useful for the purpose [12],... 

Del Moral, P. Diez, M.T. Resines, J.A. Bravo, I.G. An n, M.J. Simultaneous measurements of creatinine and purine derivatives in ruminant s urine using ion-pair HPLC. J. Liq. Chromatogr. Rel. Technol. 2003, 26 (17), 2961 2968. Patel, A.K. Sharma, P.S. Prasad, B.B. Development of a creatinine sensor based on a molecularly imprinted polymer-modified sol-gel film on graphite electrode. Electroanalysis 2008, 20 (19), 2102-2112. 

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