Molecular genetics, application

Herzberg, M. (1984) Molecular genetic probe, assay technique, and a kit using this molecular genetic probe. European Patent Application, 0128018. 

Xia X, Bollinger J, Orgam A (1995) Molecular genetic analysis of the response of three soil microbial communities to the application of 2, 4-D. Mol Ecol 4 17-28... 

Genetic applications of molecular technologies have different concerns than routine clinical laboratory testing because results are used in a predictive manner (e.g., the probability that a gene is present and, if present, whether it will be expressed and to what extent of severity). Therefore, validation procedures may differ, depending on whether the test will be used in high-risk situations or in the general population. 

Towards the end of the twentieth century, interest in biochemistry has shifted to molecular genetics and its widespread applications. The study of metabolic pathways has become a relatively small part of the subject. 

Seeger, S., Monajembashi, S., Hutter, K.-J., Futterman, G., Wolfrum, J and Greulich, K. O. (1991) Application of laser optical tweezers in immunology and molecular genetics. Cytometry 12,497-504. 

It is a truism that gene frequencies vary by ethnicity. Polymorphisms are not meaningful unless they can be studied in the context of the ethnic group that harbors them. In the case of risk reduction statistics, for example, it is well known that Jewish Ashkenazim are at 1/27 risk of carrying Tay-Sachs disease. But what is often not understood by the naive observer is that their risk can be reduced to 1/1250 through the prudent application of molecular genetic testing. On the other... 

Jaeger, K.E. and S.Wohlfarth. (1993) Bacterial lipases Biochemistry, molecular genetics and applications in biotechnology. Bioengineering, 9, 39-46. 

Fig. 11.13. Reprinted with permission of John Wiley Sons, Inc. 1990 from Van Dilla M, et al. (1990). Applications of flow cytometry and sorting to molecular genetics. Melamed MR, et al. (eds). Flow Cytometry and Sorting. New York Wiley-Liss, pp 562-604.

Describe how recent advances in molecular genetics have contributed to our knowledge of the urea cycle. List some applications of these advances to medical... 

Jennewein, S. and Croteau, R. (2001) Taxol bioynthesis, molecular genetics, and biotechnological applications. Appl. Microbiol. Biotechnol., 57,13-19. 

Microsomes prepared from maize (Zea mays) embryos or seedlings have proved to be an excellent biochemical system to study sterol bios)mthesis in vitro. The development of molecular genetics tools, the availability of specific Arabidopsis thaliana mutants and the possibilities to genetically transform these mutants allowed the application of a new strategy to study sterol biosynthesis pathways (Benveniste, 2004). Some important results of both approaches are summarized above (Figs 6.2 and 6.3). 

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