Modifiers, analysis

C. BSC-PEG (1 g, 0.4 mequiv. SC-groups) was added to a stirred solution of BSA (100 mg, 1.5 x 10 6 mol) in 0.1 M sodium borate, pH 9.2. Sodium hydroxide (0.5 N) was used to maintain pH 9.2 for 30 min. The excess of free PEG was removed by diafiltration and the product assayed for the number of free amino groups. Approximately 48 of the amino groups of the native BSA were modified. Analysis of the product by HPLC (Gel Filtration) indicated that over 65% of PEG-BSA was in an intermolecularly crosslinked form and about 35% of the product had the same molecular weight as PEG-BSA from the above example. 

Thus, with this modified analysis the circulation contribution is proportional to E, in agreement with the Bueche, De Gennes and Graessley results. However, now a power of molecular weight is lost in the viscosity,... 

Figure 37. Arrhenius plot for the degradation of vitamin A obtained by a modified analysis according to Eq. (2.75). The upper and lower curves represent 95% significance limits. (Reproduced from Ref. 314 with permission.)...

The cache notifies observer (result viewer extension) of new or modified analysis results. For... 

Olsen JV, Maeek B, Lange O, Makarov A, Homing S, Matm M. Higher-eneigy C-trap dissociation for peptide modifieation analysis. Nat Methods. 2007 4 709-12. 

When applying the results of the kinetic theory of polyatomic gases, one has to take into account the influence of vibrational and sometimes hindered rotational modes on the transport properties. The kinetic theory of molecules possessing vibrational modes of motion is still in its formative stages and is nowhere near as well-developed as that of rigid rotor molecules (see Section 14.2). Thus, the type of analysis that has been carried out for nitrogen is not possible in its entirety for polyatomic fluids. Nevertheless, a modified analysis, based on an empirical extension of the kinetic theory of diatomic gases, turned out to be possible (Hendl et al. 1991,1994 Vesovic et al. 1994). 

Requirements are captured from stakeholders, users, and subject matter experts as early as possible in the Concept stage of the life cycle (Figure 2.1). These requirements must be analyzed, quantified, and verified, and must be able to be modified. Analysis is done to guarantee clarity, consistency, and traceability to lower-level requirements and across system parameters. Quantification allows costs, schedules, and resources to be identified for requirements. Verification of requirements allows the creation of test plans that will prove out safety cases and satisfy fail-safe... 

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