Modified Yeast

Heering HA, Williams KA, de Vries S, Dekker C. 2006. Specific vectorial immobilization of oligonucleotide-modified yeast cytochrome c on carbon nanotubes. Chem Phys Chem 7 1705-1709. 

Yeasts have also been used to detoxify the diet of mycotoxins. Modified yeast cell wall mannanoligosaccharide (MOS) has been reported to effectively bind aflatoxin and to bind ochratoxin and the fusariotoxins to a lesser degree. This product has advantages over other binding agents in that it does not bind vitamins or minerals (Devegowda et al., 1998). 

AM greatly modifies the composition of the basic medium (must coming from the berries progressively crushed during the first step). It likely stimulates and modifies yeast and bacterial metabolisms, probably affecting the organoleptic characteristics of the wine. 

The emulsifying capacity of the yeast proteins was progressively improved with the extent of succinylation (Table IX) as measured by the turbidimetric technique (89). The modified yeast proteins had excellent emulsifying activities compared to several other common proteins. McElwain et al. (88) observed that succinylation of yeast protein increased emulsion viscosity but decreased emulsion stability. 

It would seem that this has little to do with SM-protein interaction profiling, and that would be true were it not for the amazing versatility of the approach. To look at two examples of how modified yeast two-hybrid approaches can potentially be used for SM screening, let us consider SM-induced protein-protein interaction inhibition (PPII) and SM-protein binding. To assess the first situation, a modified two-hybrid assay involves a lethal reporter gene and a protein pair that is known to interact (Fig. 2-3). Now, an array of SMs potentially causing PPII... 

Production of mammalian proteins (biopharmaceuticals) from genetically modified yeasts... 

Osmo and high ethanol tolerant genetically modified yeast strains IMTECH, Chandigarh Patents applied for Indian748 Del/93 749/Del/93 and 1718/Del/94 US Pat 5693526... 

Advantageously, modified yeasts can be used to reduce the t) i-cal odor of yeast, such as partially hydrolyzed yeasts (14). 

Hammond, J. R. M. (1995). Genetically-modified yeast for the 21st century. Progress to date. Yeast, 11, 1613-1627. 

Suominen P, Aristidou A, PentUUa M, Ihnen M, Ruohonen L, Koivuranta K, Robert-Perez K. (2008). Genetically modified yeast of the species Issatchenkia orientalis and closely related species and fermentation processes using same. European Patent Application Pubheation EPl 885842 A2. 

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