Mixtures Arne Tiselius

Adsorption Analysis of Amino Acid Mixtures Arne Tiselius... 

The third test of protein homogeneity, developments from which remain in common use, was that of electrophoresis. Arne Tiselius had been a research assistant in Svedberg s laboratory. From 1925 he pioneered the application of electrophoresis to the analysis and separation of protein mixtures, showing with dialyzed serum differences in mobility of the protein components and the presence of three classes of globulins, a, B, and y. 

The first important experiments on electrophoresis were carried out by the Swedish physical chemist Arne Wilhelm Kaurin Tiselius (1902-1971), who in 1937 made electrophoresis a powerful technique for studying mixtures of proteins. He devised a special type of U-tube, shown schematically in Figure 11.20, along which the protein molecules move under the influence of an electric potential. Different proteins will move at different speeds. The tube consisted of portions fitted together at ground-glass joints, so that one of a mixture of proteins could be isolated in one chamber. Optical methods are used to determine the quantity of each protein present in the mixture. 

---