Mitochondrial targeting signals intermembrane space proteins

Prediction of mitochondrial targeting signals is not an easy task. The proposed amphiphilic nature is not clear enough. Nakai and Kanehisa (1992) developed a simple method based on the amino acid composition of the segment of most amino-terminal 20 residues. In addition, a simple rule to discriminate the bipartite signal of intermembrane-space proteins was also included in PSORT. 

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