Mitochondrial assays assay

Reisch AS, Elpeleg O. Biochemical assays for mitochondrial activity assays of TCA cycle enzymes and PDHc. Methods Cell Biol. 2007 80 199-222. 

The toxicologist can still use of in silico technology36 to assess the chemical-based toxicity potential of various structural series and many molecular methodologies can still be applied, including covalent binding assays, mitochondrial assays and oxidative stress assays.29-37... 

Our observations appear to be consistent with Landolt s findings, in which they indicated that acetogenins with the bis-adjacent THF ring or the bis-nonadjacent THF ring are about ten times more active than those with the mono-THF ring after a more complete research in screening 20 acetogenins by mitochondrial inhibition assay in 1995 [81]. 

Based on the structure of dioxapyrrolomycin and related compounds, it was postulated that the insecticidal activity of these compounds was due to the uncoupling of oxidative phosphorylation. This was subsequently confirmed through mouse-liver mitochondrial assays [6]. 

Our first attempt was to synthesis some libraries, where we replaced the Z-double bonds with benzene rings, and the complex side chain with simpler groups. Typical examples were Compounds 8 and 9, (Figure 3). All the compounds were tested on a cascade consisting of the beef heart mitochondrial respiration assay and application to fimgi on leaf disks, with the most active compounds then being tested on fimgi on small plants. Unfortunately, none of the compounds showed activity in any of these tests. 

Hynes J, Nadanaciva S, Swiss R, Carey C, Kirwan S, WiU Y (2013) A high-throughput dual parameter assay for assessing drug-induced mitochondrial dysfunction provides additional predic-tivity over two established mitochondrial toxicity assays. Toxicol In Vitro 27, 560-9. 

In 2008, Filipovska and coworkers reported a comparison between cationic and neutral gold(I) complexes as potential anticancer drugs based on the results obtained from the mitochondrial assay [10]. 

Cell culture testing is used widely in vitro to evaluate the biocompatibihty of HA samples. For this, several osteoblast cell lines have been developed to assess biomaterials performance in vitro, including a murine osteoblastic cell hne (MC3T3-El) [121], a human osteoblastic precursor cell line (OPCl) [122] and a human osteoblast cell line (hFOB 1.19) [123]. Osteoblast cells interact differently with material structures, depending on the combinations of chemical, structural and environmental variables. The biocompatibility of HA surfaces can be assessed using ceU-material interaction studies such as attachment, proliferation and differentiation. Cell proliferation on HA samples is normally investigated with a mitochondrial (MTT) assay, which permits the quantitative estimation of the number of living cells on a material. 

Before an antiviral agent becomes a drug, advanced toxicity testing, pharmacological combination, and drug-interaction studies are needed. The use of new cell-based assays that can predict mitochondrial toxicity, lactic acidosis, peripheral neuropathy, anemia, hypersensitivity, lipodystrophy, and other potential side effects can alleviate these issues (Stuyver et al. 2002). 

Intracellular Enzymes Stem Cell Isolation Cellular Membrane Potential Mitochondrial Membrane Potential Gene Reporter Assay Gene Silencing (siRNA)... 

Hassoun et al. (1993) examined the effects of various pesticides on lipid peroxidation and DNA single strand breakage in the hepatic cells of female Sprague-Dawley rats. Animals were dosed orally once with endrin at 4.5 mg/kg, lindane at 30 mg/kg, chlordane at 120 mg/kg, or DDT (dichlorodiphenyl trichloro-ethane) at 40 mg/kg, or vehicle only (com oil, control). At 6, 12, and 24 hours post-dosing, 4 animals from each group were sacrificed, their livers removed, and prepared for lipid peroxidation assay. Lipid peroxidation was measured calorimetrically by determining the amount of thiobarbituric acid reactive substances (TBARS) formed. Exposure to endrin resulted in a 14.5% increase in hepatic mitochondrial... 

Assay of Subcellular Fractions for Aldrin Epoxidation. Mitochondrial and microsomal pellets were resuspended in Tris-HCl buffer. Each 5 ml incubation mixture contained the following ... 

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