Milled wood lignin preparation

Its nature as a lignin component is confirmed by its elemental composition and IR-spectrum compared with corresponding data for a milled wood lignin preparation, Table I and Figure 12. 

Lundquist K, Simonson R, Tingsvik K (1979) On the occurrence of carbohydrates in milled wood lignin preparations Sven Papperstidn 82 272-275... 

K Lundquist, R Simonson, KTingsvik. Studies of hgnin-carbohydrate linkages in milled wood lignin preparations. Svensk Papperstidning 82 452-454, 1980. 

Lignin Samples. Ball-milled (BM) aspen lignin was prepared following the procedure of Lundquist et al. (21). The yield of purified milled wood lignin obtained was usually about 10% w/w that of ethanol/benzene-extracted aspen wood. 

Our constitutional scheme is based on different kinds of information. The first type of information comes from an analytical study of milled spruce wood lignin prepared according to Bjorkman (3). The analytical data considered comprise the elemental composition of lignin, its content of methoxyl groups and other ether bonds, the types and amount of its different hydroxyl groups, carbonyl, and lactone groups, and the kind and number of its biphenylyl linkages and other bonds in which the benzene nucleus is involved. The work that led to data of this kind has been carried out in various laboratories and has been described previously (9,11). 

Besides cellulolytic enzyme lignin, the so-called Bjorkman lignin, alternatively referred to as "milled wood lignin" (MWL) is the best preparation known so far, and it has been widely used for structural studies. When wood meal is ground in a ball mill either dry or in the presence of nonswelling solvents, e.g., toluene, the cell structure of the wood is destroyed and a portion of lignin (usually not more than 50%) can be obtained from the suspension by extraction with a dioxane-water mixture. MWL preparations always contain some carbohydrate material. 

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