Migration track assays

ECM migration tracks. The assay is based on the immunohisto-chemical detection of ECM components deposited on the substratum by the cells while migrating (Bade and Nitzgen, 1985 Bade and Feindler, 1988). Laminin (Bade and Nitzgen, 1985), fibronectin (Bade and Feindler, 1988 Seebacher et al., 1988), plasminogen activator inhibitor-1 (PAI-1 Seebacher et al., 1992) have been used as ECM track markers. 

Cell migration was assayed by automated time-lapse video microscopy. In this technique, cells are imaged on the surfaces and the individual cell tracks are reconstructed from the position of each cell at successive time points. The automation enables time lapse videos to be taken from multiple locations on the same surface at the same time. Thus, the entire gradient can be imaged at once. This high-throughput technique combined with the combinatorial sample preparation enables the exploration of a relatively large parameter space quickly. 

Checkerboard analysis. The filter assay does not allow one to directly observe migration of cells, and track them to establish... 

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