Microwave bags

Nylon microwave and roasting bags were reported to release, at cooking temperatures, volatile compounds, such as Nylon 6,6 cyclic monomer and cyclic oligomers up to the tetramer, and Nylon 6 monomer and cyclic oligomers up to the octamer. The same non-volatile compounds (except Nylon 6 heptamer and octamer) were extracted from the same packages (Soto-Valdez et al., 1997). 

Soto-Valdez, H., Gramshaw, J.W. and Vandenburg, H.J. (1997). Determination of potential migrants present in Nylon microwave and roasting bags and migration into olive oil, Food Addit. Contam., 14, 3, 309-318. 

One has to note, however, that other polymer composite materials also popular in solid-phase synthesis, for example, polyethylene or polypropylene tea bags such as IRORI kans, lanterns, crowns, or plugs are generally less suitable for high-temperature reactions (>160°C). Therefore, microwave irradiation is not typically a very suitable tool to speed up reactions, which utilise these materials as either a solid support or as containment for the solid support. 

Packaged food containers are sometimes coated with PFCs (perfluorochemicals, the same stuff that s in Teflon) to prevent leaking. If you buy these, take the food out of the box and place it in glass or ceramic jars. Microwave popcorn often comes in PFC-coated bags. Stick to good old-fashioned do-it-yourself popcorn instead. 

Gramshaw, J.W., Soto-Valdez, H., 1998, Migration from polyamide microwave and roasting bags into chicken. Food Add. Contam. 15, 3, 329-335. 

Equation 8.4) 1/ — = constant T You probably have some familiarity with this law. Perhaps you have observed a balloon expanding on a hot day or a bag of microwave popcorn swelling as the contents begin to heat up. 

Microwave oven. Be sure your soil is moist and free of stones and metallic particles. Put it in a polypropylene baking bag or a microwave-safe mixing bowl-in either case, the container should not be tightly sealed. At low power (30-40 percent), adequate heating may take 10-15 minutes ot operation. At full power, heating will take about Th minutes for a 2-pound batch. If you see any sparks, stop the process immediately this indicates that metallic particles are present in the soil. In this case, use another method. 

PET Polyethylene terephthalate Beverage bottles, frozen food, boil-in-bag pouches, microwave food trays... 

A high speed hybridization procedure (total, including fixation and detection, 1 h) has been described by Bourinbaiar et al. (1991). They suspended washed lymphocytes, for the detection of HIV, in a mildly hypotonic medium (1 part medium and 2 parts distilled water) and spread 25 xl ( 10 cells) in 8 mm wells of Teflon-coated multiwell glass slides and fixed the cells in Carnoy s II solution (60% methanol, 30% chloroform, 10% glacial acetic acid) for 10 min at room temperature and then for 10 min in absolute acetone. Five microliters of biotinylated probes (100 ng/ml) is added to the dried cells and the slides are covered with autoclavable plastic (cut from biohazard bag) and placed in a microwave oven. The position of the slide in the oven and the incubation time are crucial for this hybridization step. In microwave ovens with a carousel, the slide is placed about 6 cm from the center and a 150 ml beaker filled with tap water is placed in the center. The slide is then irradiated for 30 s on Defrost . The probes can be detected after a rinse with 2 x SSC. 

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