Microsomes membrane

Fig. 1. Relative composition of root microsomal membranes from 24 land races, varieties and breeding lines of rice which differ in their salt resistance. Campesterol, Stigmasterol and Sitosterol as % of total sterols 16 0, 18 1, 18 2 and 18 3 fatty acids as % of total fatty acids Na transport on a relative scale from (1) lowest to (9) highest. Data of D.R. Lachno, T.J. Flowers A.R. Yeo (unpublished).

The mechanisms involved in the establishment of lipid asymmetry are not well understood. The enzymes involved in the synthesis of phospholipids are located on the cytoplasmic side of microsomal membrane vesicles. Translocases (flippases) exist that transfer certain phospholipids (eg, phosphatidylcholine) from the inner to the outer leaflet. Specific proteins that preferentially bind individual phospholipids also appear to be... 

Liebler, D.C. et al.. Antioxidant actions of beta-carotene in liposomal and microsomal membranes role of carotenoid-membrane incorporation and alfa-tocopherol, Arch. Biochem. Biophys., 338, 244, 1997. 

A full understanding of the role of pectin in plant development requires elucidation of the mechanisms that regulate p>ectin biosynthesis (6). Our strategy for studying the biosynthesis of HGA was to 1) establish a PGA-GalAT assay that would allow detection of synthesized HGA, 2) characterize the enzyme in microsomal membranes, 3) characterize the product synthesized by the enzyme in microsomal membranes, and 4) solubilize the enzyme and characterize the solubilized enzyme and its product. 

CHARACTERIZATION OF THE PRODUCTS SYNTHESIZED BY PGA-GALAT IN MICROSOMAL MEMBRANES... 

Walter, P., and Blobel, G. (1981a). Translocation of proteins across the endoplasmic reticulum III. Signal recognition protein (SRP) causes signal sequence-dependent and site-specific arrest of chain elongation that is released by microsomal membranes. J. Cell Biol. 91, 557-561. 

Walter, P., and Blobel, G. (1983). Preparation of microsomal membranes for cotranslational protein translocation. Methods Enzymol. 96, 84—93. 

Chelomin, V.P. and N.N. Belcheva. 1992. The effect of heavy metals on processes of lipid peroxidation in microsomal membranes from the hepatopancreas of the bivalve mollusc Mizuhopecten yessoensis. Comp. Biochem. Physiol. 103C 419-422. 

Devineau, J. and C. Amiard Triquet 1985. Patterns of bioaccumulation of an essential trace element (zinc) and a pollutant metal (cadmium) in larvae of the prawn Palaemon serratus. Mar. Biol. 86 139-143. Dib, A., J.P Clavel, and J.P. Carreau. 1989. Effects of gamma-linolenic acid supplementation on lipid composition of liver microsomal membranes. I. Pregnant rats fed a zinc-deficient diet and those fed a balanced one. Jour. Clin. Biochem. Nutr. 6 95-102. 

Levin RM, Levin SS, Zderic SA, Saito M, Yoon JY, Wein AJ 1994 Effect of partial outlet obstruction of the rabbit urinary bladder on ryanodine binding to microsomal membranes. Gen Pharmacol 25 421 125... 

Fath A, Boiler T. Solubilization, partial purification and characterization of a binding site for a gly copeptide elicitor from microsomal membranes of tomato cells. Plant Physiol 1996 112 1659-1668. 

Litman etal. [ 391 analyzed a set of 34 diverse drugs and their effect on the kinetics of the ATPase activity of the microsomal membrane fraction of a P-gp overexpressing CHO cell line. They found a correlation (r=0.75) between the affinity of the modulators and their van der Waals surface area, while the affinity data did not correlate with the lipophilicity of the compounds, expressed as calculated octanol/ water partition coefficients. 

Kuroiwa, T., Sakaguchi, M., Mihara, K., and Omura, T. (1991). Systematic analysis of stop-transfer sequence for microsomal membrane. Biol. Chem. 266, 9251—9255. Kuroiwa, T., Sakaguchi, M., Omura, T., and Mihara, K. (1996). Reinitiation of protein translocation across the endoplasmic reticulum membrane topogenesis of multispan-ning membrane proteins. J. Biol. Chem. 271, 6243—6248. 

Imai, Y. and Sato, R. Evidence for two forms of P-450 hemo-protein in microsomal membranes. Biochem. Biophys. Res. Commun. (1966) 23 5-11. 

Figure 5.1. Representations of double-exponential and bimodal Lorentzian distribution analyses of DPH fluorescent decay lifetimes in liver microsomal membranes. Results (see Table 5.2) are normalized to the major component. The double-exponential analysis, represented by the vertical lines, recovers lifetimes near the centers of the Lorentzian distributions. The width of the distributions represents contributions from a variety of lifetimes. (From Ref. 17.)...

Three isoenzymes of carboxylesterase were purified from rat liver micro-somes and were named RL1, RL2, and RH1. These differ from each other in their response to hormone treatment, inducibility, substrate specificity, and immunological properties [75], It was shown that RL1, RL2, and RH1 resemble hydrolases p/ 6.2/6.4, pI 6.0, and pI 5.6, respectively. Enzyme RL2 was found to be identical to egasyn, a protein with esterase activity found in the endoplasmic reticulum [76], The role of egasyn is to stabilize glucuronidase (EC 3.2.1.31) by noncovalent binding to the microsomal membrane. 

CYP2D protein is present in rat brain mitochondrial and microsomal membranes (Miksys et al., 2000). 

H5. Hanninen, 0., and Puukka, R., Effect of digitonin on UDP-glucuronyltransferase in microsomal membranes. Suom. Kemistilehti B 43, 451-456 (1970). 

Figure 3. Gel filtration of alkali-soluble xyloglucan on columns (95 x 1.5 cm) of Sepharose CL-6B. Pea microsomal membranes were incubated for various periods with GDP-[14C]fucose and unlabelled sugar nucleotides. Products were eluted with 0.1 M NaOH in 1 ml fractions. Molecular weights of dextran markers, 1 = 264000 D 2 = 70000 D 3 = 40000 D 4 = 10600 D Glc=glucose. Redrawn from Camirand and Maclachlan (20).

---