Microscopy, optical staining

At low temperatures, partial co-crystallisation was indicated by transmission electron microscopy and differential scanning calorimetry [156, 157]. Both electron microscopy of stained sections and optical microscopy showed that the segregated low molar mass material was present as small domains between the stacks of dominant lamellae within the spherulites/ax-ialites [115, 157, 158],... 

Electron and optical microscopes are being used to see blend homogeneity. Elastomer-plastic blends are somewhat easier to identify than elastomer-elastomer blends because normal staining techniques, e.g., osmium tet-raoxide, can be used in the case of plastic-elastomer blends. Normally, there are two methods that are followed for examining the blend surface by electron microscopy. 

Confocal laser scanning microscopy (CLSM) in conjunction with specific staining techniques is best suited to elucidate intracellular trafficking and localization. CLSM is a specific epifluorescence microscopical technique capable of optical cross-sectioning with a spatial resolution of 1 /urn and below [41, 42],... 

After polymerization was complete, transmission electron microscopy was carried out on thin sections of the 10/90 and 20/80 compositions. Confirming the optical micrographs, the polystyrene phase was continuous for the fully reacted product. As illustrated in Figure 5 for the 10/90 system, the oil phase (stained dark) contains a considerable amount of occluded polystyrene. For the 20/80 system, data not shown, dual phase continuity was found. The polystyrene phase was relatively pure, but the oil-rich phase had much occluded... 

MICROSCOPY tChemical). Use of a microscope primarily for study of physical struclure and identification of materials. This is especially useful in forensic chemistry and police laboratories. Many types of microscopes are used in industry most important arc the optical, ultra-, polarizing, stereoscopic, electron, and X-ray microscopes, Organic- dyes of various types are used to stain samples for precise identification. 

In this paper we describe two types of observations on egg-yolk lecithin. We present the results of our study of homeotropically oriented samples of La phases by polarizing optical microscopy. This study provides evidence, amid apparently nonsimilar aspects, of the existence of an elementary typical object which we have interpreted as a dislocation. We also studied thin samples of stained lecithin in the high vacuum of the electron microscope. In addition to the defects that are typical of this type of sample, we observed the same elementary object as in La lecithin. 

Fig. 13.2. Preferred association of LCM with tumor cells in vivo. Vibratome sections of brain of rats bearing 9L tumor that have been injected i.v. with diO-LCM, and sacrificed 2 min later. Sections in panels A and C were stained with TR-conjugated WGA. Specimens in panels B and C were observed by fluorescence microscopy using fluorescein optics. The scale bar represents 100 pm. (Taken from ref. 531.)...

---