Microorganisms, application

A set of Saccharomyces cerevisiae reductases was screened in collaboration with J. D. Stewart s group (University of Florida). Itwas demonstrated that diketo ester la is accepted as substrate by at least three different NADP(H)-dependent reductases of this microorganism. Application of a cell-free system in preparative batches using enzyme-coupled coenzyme regeneration afforded (R)-2a with more than 99% enantiomeric excess [13]. 

Natural product Producing microorganism Application, target... 

Stirling, D., CoDins, A.N., Sheldrake, G.N., and Crosby, J. (eds) (1992) The use of aminotransferases for the production of chiral amino adds and amines, in Chirality in Industry, John Whey Sons, Ltd, Chichester, UK, 209-222. n Shin, J. and Kim, B. (1997) Kinetic resolution of (S)-mefhylbenzylamine with (o-ammotransferase screened from soil microorganisms application of a bi-phasic system to overcome product... 

Commercial applications for polysaccharides include their use as food additives, medicines and industrial products. Although plant polysaccharides (such as starch, agar and alginate) have been exploited commercially for many years, microbial exopolysaccharides have only become widely used over the past few decades. The diversity of polysaccharide structure is far greater in micro-organisms compared to plants and around 20 microbial polysaccharides with market potential have been described. However, microorganisms are still considered to be a rich and as yet underexploited source of exopolysaccharides. 

Microorganisms have been identified and exploited for more than a century. The Babylonians and Sumerians used yeast to prepare alcohol. There is a great history beyond fermentation processes, which explains the applications of microbial processes that resulted in the production of food and beverages. In the mid-nineteenth century, Louis Pasteur understood the role of microorganisms in fermented food, wine, alcohols, beverages, cheese, milk, yoghurt and other dairy products, fuels, and fine chemical industries. He identified many microbial processes and discovered the first principal role of fermentation, which was that microbes required substrate to produce primary and secondary metabolites, and end products. 

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