Microinjection equipment

A detailed discussion of suitable microinjection equipment and technical aspects is beyond the scope of this chapter and are described in ref. 3. The glass micropipettes used for penetration of cells and delivery of the sample are avail-... 

D invasion of microinjection-derived spheroids in collagen 3-D from 3-D Reproducible, automated, adaptable to other ECM components or fresh tumor biopsy material. 96-well system containing multiple spheroids/weU can be used in HT Specialist equipment needed, multiple spheroids/weU can potentially lead to secreted chemo-attractants (33)... 

The ES cell aggregation method, on the other hand, is a relatively easy technique, and does not need expensive equipment or extensive experience, but demands higher quality ES cells (early passage cells are required). This method was developed by Nagy et al. (27). After microinjection or aggregation, blastocysts are transferred into the uterus of pseudopregnant mice using standard procedures (26). 

Cell microinjection is a very delicate technique, requiring considerable expertise, that can hardly be conveyed by a mere description of the technique itself. Since this is not within the scope of this book, the interested reader is thus referred to more comprehensive reviews, describing in details various equipment and protocols (Graessman et al., 1980 Wadsworth, 1999). Here, we will only give a general description of the application relative to the study of gap junctional communication between cells. 

Stephens, D. L., Miller, T. J., Silver, L., Zipser, D., and Mertz, J. E. (1981). Easy-to-use equipment for the accurate microinjection of nanoliter volumes into the nuclei of amphibian oocytes. Analy. Biochem. 114, 299-309. 

Micro-milling of tool-steel microinjection molds Machining of microsurgical equipment Machining of optical glass and fibers components Machining of metal and ceramic matrix composite components... 

Microinjection Molding Machines, Auxiliary Equipment, and Polymer Materials... 

Development of auxiliary equipment and technologies to improve the microinjection molding process, such as variotherm mold heating systems, vacuum venting, alignment techniques, etc. 

Fig. 5. A typical arrangement of the equipment needed for the microinjection of fertilized one-cell eggs. A Agla micrometer S5ringe. B Liquid-paraffin-filled tube. C Left-hand micromanipulator. D Inverted microscope. E Base plate. F Camera (optional). G Left-hand instrument tube for holding pipet. H Microinjection chamber (depression slide) sitting on fixed stage. I Right-hand instrument tube for injection pipet. J Air-filled tube. K Glass 50-mL syringe. L Right-hand micromanipulator. M Video system (optional).

Microinjection is a light microscope based technique. The microinjection capillary and its positioning relative to cells are tracked by phase contrast. Moreover, phase contrast is used to identify cell nucleus versus cytoplasm. Microinjection requires in addition to an inverted phase contrast microscope at least two committed pieces of equipment (Fig. 1). These are a micromanipulator to position the injection capillary and a pressure regulator to increase pressure to the capillary selectively as it... 

Let me be straightforward microinjection, even with present state-of-the-art semi-automatic injection equipment is a skill that does require a time investment. Based on my experience of having taught microinjection to 15 people in a research laboratory setting over the last several years, almost anyone can do acceptable microinjection for research purposes with a week of learning. However, true skill, in being able to handle a... 

This chapter discusses protocols for fluorescent labelling of antibodies, optimized fixation, and permeabilization of cells, and describes several immunostain-ing procedures with the aim to quantitatively anafyse the localization of antigens in fixed and living cells. Various aspects of the imaging equipment presently available, and the ways how to most efficiently use it in order to quantify and document results will be discussed. Labelling in living cells by microinjection of fluorescently labelled antibodies and subsequent time-lapse microscopy will also be discussed. 

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