Microfabricated fluidic devices

Microfabricated fluidic devices (microchips) are potentially useful for multidimensional separations because high-efficiency separations can be achieved and small sample volumes can be manipulated with minimal dead volumes between interconnecting channels. Electro-kinetically driven separation techniques demonstrated on microchips include... 

Rocklin, R. D., Ramsey, R. S., and Ramsey, J. M. (2000). A microfabricated fluidic device for performing two-dimensional liquid-phase separations. Anal. Chem. 72, 5244-5249. 

FIGURE 7.41 Picture of the microfabricated fluidic device integrated with a standard MALDI-TOF sample plate. Because of the self-activating character of the microfluidic device, the system can be introduced into the MALDI ionization chamber without any wire or tube for the sample introduction and the flow control [820]. Reprinted with permission from the American Chemical Society. 

Culbertson, C.T., Alarie, J.P., McClain, M.A., Jacobson, S.C., Ramsery, J.M., Rapid cellular assays on microfabricated fluidic device. Micro Total Analysis Systems, Proceedings 5th [iTAS Symposium, Monterey, CA, Oct. 21-25, 2001, 285-286. 

The Coupling of Microfabricated Fluidic Devices with Electrospray Ionization Mass Spectrometers... 

Interfacing Microfabricated Fluidic Devices to the Outside World... 

These techniques represented our full attempts at connecting microfabricated fluidic devices to the outside world. We had pushed as far as we could with our limited engineering resources in order to develop the interface. At that point, it was time for industries to also get into the game of coupling microfabricated devices to mass spectrometers. 

Rocklin RD et al (2000) A microfabricated fluidic device for performing two-dimensional liquid-phase separations. Anal Chem... 

Many individually microfabricated fluidic devices have been demonstrated and even marketed commercially. As sample volumes become smaller, and the analysis of individual cells or molecules is desired, the research instra-mentation itself decreases in size and new methods of interconnection and assembly are required. Microfluidic corrponents must often first be assembled from smaller subunits (e. g., channels, actuators) and then further assembled together to form microfluidic systems and instrumen-... 

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