Microcolumn HPLC

Also, SEC-cSFC has been reported [947]. Due to the nature of the interface and the use of conventional-sized SEC columns, only small portions of the SEC fractions can be analysed by cSFC (i.d. <100 xm) packedcapillary SFC would be an obvious improvement to avoid overloading. Fujimoto el al. [931] have reported the combined power of [iSEC-TLC-DRIFT (in situ). Because of the reduced flow-rate, microcolumn HPLC has great promise for this type of coupling. 

Opiteck, G.J., Jorgenson, J.W., Moseley, M.A. Ill, Anderegg, RJ. (1998). Two-dimensional microcolumn HPLC coupled to a single quadrupole mass spectrometer for the elucidation of sequence tags and peptide mapping. J. Microcolumn. Sep. 10, 365-375. 

Fig. 4 Comparison of tryptic digests from (A) phosphorylated and (B) dephosphorylated forms of /3-casein, separated by microcolumn HPLC. Arrows point to the two peaks that exhibit different retention times between the two forms. Each chromatogram was obtained from a 300-ng protein sample.

Phenols cresols — Reverse phase microcolumn HPLC — 0.5pg L1 [68]... 

In conclusion, the higher column efficiencies available with microcolumn HPLC could prove to be an absolute requirement for the analysis of complex biological samples. Technical advances in the... 

Detectors provide another route to optimizing selectivity by enhancing detectability of various classes of compounds. Discussion is included that describes the noise sources in optical detectors that allow optimization of such detectors. Two additional chapters deal with new and potential detectors including laser fluorimetry for HPLC, microcolumn HPLC, and FIA. 

---