Microbial glycosidases

Park CS, Yoo MH, Noh KH, Oh DK (2010) Biotransformation of ginsenosides by hydrolyzing the sugar moieties of ginsenosides using microbial glycosidases. Appl Microbiol Biotechnol 87 9-19... 

Hydrolytic reactions are catalyzed by extracellular hydrolases and mineral surfaces (Chrost, 1990 Hoffman, 1990). For enzymatic reactions, a defined substrate or moiety must match the catalytic site of a specific enzyme. The most studied examples in aquatic systems are glycosidases, peptidases, and phosphatases (Munster and De Hann, 1998 see Chapter 13). In general, hydrolytic reactions break the relatively labile C—N and C—O bonds that link monomers, generating lower molecular weight products more suitable for microbial consumption. 

The most widely used intestinal microbial strains used to test colon targeting and enzymatic biodegradation of the carrier, respectively, are microorganisms with azoreductases and different types of glycosidases. 

Hudlicky, T, Rouden, J, Luna, H, Allen, S, Microbial oxidation of aromatics in enantiocontrolled synthesis. 2. Rational design of aza sugars (emio-nitrogenous). Total synthesis of (-l-)-kifunensine, mannojirimycin, and other glycosidase inhibitors, J. Am. Chem. Soc., 116, 5099-5107, 1994. 

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