Microbes identification

The FDA, USDA, and EPA have established methods and standards for detecting food- and water-borne pathogens these methods are available on the agencies websites [4—81. Many of the procedures for microbe identification rely on culturing the sample for subsequent identification by multiple tests such as colony characteristics, selective growth conditions, and biochemical assays for metabolites. Such traditional methods take upward of 24 h and remain the standard against which new... 

The methods involved in the production of proteins in microbes are those of gene expression. Several plasmids for expression of proteins having affinity tails at the C- or N-terminus of the protein have been developed. These tails are usefiil in the isolation of recombinant proteins. Most of these vectors are commercially available along with the reagents that are necessary for protein purification. A majority of recombinant proteins that have been attempted have been produced in E. Coli (1). In most cases these recombinant proteins formed aggregates resulting in the formation of inclusion bodies. These inclusion bodies must be denatured and refolded to obtain active protein, and the affinity tails are usefiil in the purification of the protein. Some of the methods described herein involve identification of functional domains in proteins (see also Protein engineering). 

Identification and Differentiation of Microbes by PyMS with ANNs... 

Unyayar A, Mazmanci MA, Atacag H, Erkurt EA, Coral GA (2005) Drimaren Blue X3LR dye decolorizing enzyme from Funalia trogii one step isolation and identification. Enzyme Microb Technol 36 10-16... 

Jagannatha, H. M., Sharma, U. K., Ramaseshan, T., Surolia, A., and Balganesh, T. S. (1991). Identification of carbohydrate structures as receptors for localized adherent enteropathogenic Escherichia coli. Microb. Pathog. 11, 259-268. 

Jebakumar Solomon RD, Subramaniam K, Jayaraj V. (2005) Isolation, identification and study of antimicrobial property of a bioactive compound in an Indian medicinal plant, Acalypha indica (Indian-Nettle). World J Microb Biotech 21 1231-1236. 

Crooks, C. and Lamb, C. (2001). Identification of a host-pathogen specific gene conferring protection from a preformed antimicrobial activity in Arabidopsis. In "10th International Congress on Molecular Plant-Microbe Interactions University of Wisconsin", Madison, USA. 

Akiyama, K., Matsuoka, H., and Hayashi, H., Isolation and identification of a phosphate deficiency-induced C-glycosylflavonoid that stimulates arbuscular mycorrhiza formation in melon roots. Mol Plant-Microbe Interact., 15, 334, 2002. 

Straney, D.C., Van Etten, H.D. Characterization of the PDA1 promoter of Nectria haematococca and identification of a region that binds a pisatin-responsive DNA binding factor. Mol Plant Microb Interact 1994 7 256-266. 

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