Methyl dependence

Karymov MA, Tomschik M, Leuba SH, Caiafa P, Zlatanova J (2001) DNA methylation-dependent chromatin fiber compaction in vivo and in vitro requirement for hnker histone. Faseb J 15 2631—2641 Kaszas E, Cande WZ (2000) Phosphorylation of histone H3 is correlated with changes in the maintenance of sister chromatid cohesion during meiosis in maize, rather than the condensation of the chromatin. J Cell Sci 113(Pt 18) 3217-3226... 

Young Jl, Hong EP, Castle JC, Crespo-Barreto J, Bowman AB et al (2005) Regulation of RNA splicing by the methylation-dependent transcriptional repressor methyl-CpG binding protein 2. Proc Natl Acad Sci U S A 102 17551-17558... 

In order to reduce a nitro-compound to an azoxy-compound, either sodium amalgam or alcoholic-caustic potash or caustic soda is used. With nitrobenzene, particularly, the reaction takes place most surely by dissolving sodium in methyl alcohol as above. The reducing action of sodium methylate depends upon the fact that it is oxidised to... 

The detection of DNA methylation genome-wide requires methylation-dependent pretreatment (31532). This is because the methylation occurs at the major groove of DNA rather than at the hydrogen carbons. Therefore, hybridization techniques are unable to distinguish between 5mC and unmethylated cytosines. In addition, amplification methods (PCR) do not... 

A common denominator for the methylation-dependent adaptation and the CheZ-dependent termination or adaptation is that both processes are triggered by changes in the phosphorylation level of CheY. It appears that the complete mechanism of adaptation, methylation-dependent and independent, is still not fully resolved. Methylation-independent adaptation was also demonstrated in B. subtilis, where it was found to be very effective [366]. 

The difference between E. coli and other species is not restricted to the presence or absence of Che proteins or to their number. Some species have Che proteins that are absent in E. coli (Table 12). For example, B. subtilis contains the protein CheV, which is homologous to both CheW and CheY and can, in part, functionally replace CheW [604], It also contains CheC and CheD, which share no significant homology with other Che proteins [605]. These proteins are likely involved in methylation-dependent adaptation of B. subtilis, which appears to be totally different from that of E. coli [606]. Two forms of CheC and a single form of CheD are also present in H. salinarium [539]. CheD appears in S. meliloti as well [268]. M. xanthus contains FrzE, which is a kind of a CheA-CheY hybrid [1], and FrzZ, which consists of two domains homologous to CheY [745]. 

The same parameters were obtained by AFM imaging of chromatin fibers reconstituted in vitro with unmethylated and methylated DNA when reconstitution was performed in the absence of linker histone. After addition of H1 histone to chromatin fiber reconstituted around methylated DNA, the degree of compaction observed was different and was very close to that observed in vivo after induction ofDNA hypermethylation. The values obtained were 0.46 for the control vs. 0.70 for the hypermethylated chromatin fibers from the in vivo experiments and 0.48 for the control vs. 0.67 for the methylated HI-containing reconstituted ones. This leads to the conclusion that HI histone is required for DNA-methylation dependent compaction of chromatin structure. ... 

Kaiymov MA, Tomschik M, Leuba SH et al. DNA methylation-dependent chromatin fiber compaction in vivo and in vitro Requirement for linker histone. FASEB J 2001 15 2631-2641. 

Fig. 2.42 Simplified reaction scheme of photosynthetic formation of organic compounds. In boxes are stable substances which are possibly emitted. The radical intermediates may undergo several competitive reactions, esp. HCO (formyl) and CH3 (methyl) depending from the ratio between reactants (H, CO, and O2). In oxidative environment, acids are formed from aldehydes.

The ambident anion of a 1,3-bisphenylmalondiamidine undergoes exclusive C- or N-methylation, depending on whether the countercation is Li or Na, respectively (5). 

Both, G. W., Baneijee, A. K., and Shatkin, A. J., 1975, Methylation-dependent translation of viral messenger RNAs in vitro, Proc. Natl. Acad. Sci. USA 72 1189. 

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