METHOXYCOUMARIN-3-CARBOXYLIC ACID

CA Index Name 2//-l-Benzopyran-3-carboxylic acid, 7-methoxy-2-oxo- 

Other Names 3-Carboxy-7-methoxycoumarin 7-methoxy-2-oxo-2//-chromene-3-carboxylic acid 7-methoxy-2//-chromene-3-carboxylic acid 7-methoxy-3-carboxycoumarin 7-methoxycoumarin-3-carboxylic acid 

Merck Index Number Not listed Chemical/Dye Class Coumarin Molecular Formula C11 Hg O5 Molecular Weight 220.18 

Physical Form White crystals white crystalline 

Solubility Soluble in acetone, acetonitrile, N,N-dimethylformamide, dimethyl sulfoxide 

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A different approach to design a self-calibrating dye was proposed [70], in which a viscosity-sensitive molecular rotor (2-cyano-3-(4-dimethylaminophenyl) prop-2-enoic acid) was covalently linked to a reference dye, 7-methoxycoumarin-3-carboxylic acid, which exhibited no viscosity sensitivity (40, Fig. 13). A ratiometric measurement, that is, rotor emission relative to reference emission, was shown to be widely independent of dye concentration [70]. However, the design of such a ratiometric dye poses some challenges because of resonance energy transfer from... 

The feasibility of the screening scheme of fluorescent RNP sensors (Fig. 10.4) was demonstrated by utilizing libraries of fluorescent RNP receptors constructed from the 29 different ATP-binding RNP, which obtained from RRE30N RNAs (Fig. 10.2a) and Rev peptides modified with various fluorophores, 7-methoxycoumarin-3-carboxylic acid (7mC-Rev), 4-fluoro-7-nitrobenz-2-oxa-1,3-diazole (NBD-Rev), and Cy5 mono NHS ester (Cy5-Rev) at the N-terminal. Complex formation of the 29 different RNA subunits and Pyr-Rev, 7mC-Rev, NBD-Rev, or Cy5-Rev afforded four independent fluorescent RNP libraries. 

Cui, Z. Preparation of 7-methoxycoumarin-3-carboxylic acid. Faming Zhuanli Shenqing CN 102924415, 2013. 

Li, J. Liu, X. Zhao, R. Xiong, S. Ma, H. Lu, Q. Xu, L. Labeling analysis of neuropeptides with 7-methoxycoumarin-3-carboxylic acid N-succinimidyl ester. Gaodeng Xuexiao Huaxue Xuebao 2006,27, 2297-2299. 

Methoxycoumarin-3-carbonyl azide 7-Methoxycoumarin-3-carboxylic acid 7-Methoxycoumarin-3-carboxylic acid succinimidyl ester Pacific Blue (6,8-Diliuoro-7-hydroxycoumarin-3-carboxylic acid)... 

Hydroxy-2-oxo-2//-1 -benzopyran-3-carboxylic acid Me ester, in H-00426 7-Methoxycoumarin-3-carboxylic acid, in H-00426... 

Reaction of 3-acetylcoumarins with Vilsmeier reagents has given propeniminium salts (154) which are useful intermediates.165 4-Diazomethyl-7-methoxycoumarin is unexpectedly stable and does not react with alcohols or phenols but with carboxylic acids, it yields... 

Alkylation of carboxylate anions by 4-bromomethyl-7-methoxycoumarin (Br-Mmc) (Figure 19) has been suggested by Dunges [459, 460] as a method for the fluorescence labelling of carboxylic acids and other compounds with acidic functions. Subsequently, a considerable number of related reagents have been suggested. 

Diazomethyl-7-methoxycoumarin is a rather stable reagent which allows convenient derivative formation carboxylic acids form esters by heating in acetonitrile. With alcohols, fluorescent ethers are obtained in dichlo-romethane in the presence of HBF4 as catalyst [477]. 

The reagents containing hydroxy group have been developed for FL derivatization of carboxylic acid or other activated acids. 1-Pyrenemethanol (1-PM), 4-hydroxymethyl-7-methoxycoumarin (HMC), and 5-(4-pyridyl)-2-thiophenemethanol (PTM) have been applied to carboxylic acids with LC-FL detection. 

Acids, carboxylic O-p-N itrobenzyl-/V, Al -diisopropyliso-urea (PNBDI) p-Bromophenacyl bromide (PBPB) 4-Bromomethyl-7-methoxycoumarin... 

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