Metabolization fungal esterase

The metabolism of taxol by Eucalyptus perriniana cell suspension cultures has been recently reported to induce hydrolyses of ester bonds at C-13, C-10 and C-2 [222]. At this moment only very few data have been published about the microbial metabolism of taxoid compounds only site specific hydrolyses of acyl side-chains at C-13 or C-10 by extracellular and intracellular esterases of Nocar-dioides albus SC13,911 and N. luteus SC13,912, respectively, have been reported [223]. On the other hand, Hu et al. [224-226] have recently described some fungal biotransformations of related natural taxane diterpenes extracted from Chinese yews or their cell cultures, in order to obtain new active substances or precursors for hemisynthesis. The taxadiene 145, a 14 -acetylated derivative... 

Also of limited practical importance is some resistance of Venturia inaequalis (causing apple scab) against kresoxim-methyl, which has its origin in the esterase-mediated metabolization of the active substance by the resistant isolates [85]. Target mutations Phel29Leu (yeast numbering) lead only to lower resistance factors in a few fungal species, and seem also to be of minor practical relevance. 

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