Metabolic studies to identify and quantify pathways in cells

Metabolic studies to identify and quantify pathways in cells 

Calculations from the data indicated that oxidative metabolism produced heat of I pW per cell. The Embden-Meyerhof pathway to furnish lactate was estimated to give 1.8 pW per cell. Using an elderly LKB model 10700-1 flow calorimeter (see Reference 28) to pump the cell suspension from a stirred vessel, the heat production was found to be 2.5 pW per cell. Compared to the calculated value of 2.8 pW per cell, the similarity was considered to be within the experimental error to justify that all the catabolic pathways had been held to account for the respiratory burst. 

There is an alternative to pumping cell suspensions through a flow calorimeter from a culture and measuring changes in the concentrations of metabolites off-line. At the microanalytical scale, it is to make on-line estimations using probes situated in the calorimetric vessel. The Thermometric TAM has a series of different batch insertion vessels with a small volume (see Reference [3]). One type of vessel is designed for tissue perfusion and has a 4- 

Flux of 2C11-12 mouse macrophage hybridoma cells calculated from the measured reaction flux and the theoretical molar reaction enthalpy for each 

These values are used to calculate the enthalpy change for complete oxidation of glutamine  

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