Membrane structure/function freeze-fracture studies

We now examine some additional results also obtained by freeze-fracture electron microscopy using thylakoid membrane fragments. The membrane fragments were obtained either by detergent fragmentation and subsequent separation by differential centrifugation or by mechanical fragmentation followed by separation by aqueous-polymer two-phase partition. These studies yielded useful and complementary information on the thylakoid-membrane structure itself as well as the functional properties associated with the particular structure. 

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