Membrane reversible

Reverse Osmosis. Osmosis is the flow of solvent through a semipermeable membrane, from a dilute solution to a concentrated solution. This flow results from the driving force created by the difference in pressure between the two solutions. Osmotic pressure is the pressure that must be added to the concentrated solution side to stop the solvent flow through the membrane. Reverse osmosis is the process of reversing the flow, forcing water through a membrane from a concentrated solution to a dilute solution to produce pure water. Figure 2 illustrates the processes of osmosis and reverse osmosis. 

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Airother interesting facet of lipid anchors is that they are transient. Lipid anchors can be reversibly attached to and detached from proteins. This provides a switching device for altering the affinity of a protein for the membrane. Reversible lipid anchoring is one factor in the control of signal transduction pathways in eukaryotic cells (Chapter 34). 

Incubate dishes with NGG-sap-PBS solution for 10 min at room temperature. The normal goat globulin serves as a blocking protein to minimize nonspecific binding, and the 0.1% saponin renders the fixed cell membranes reversibly permeable to proteins (see Notes 4 and 5). 

Purification and separation—solvent extraction, ion exchange, cementation, adsorption, liquid membranes, reverse osmosis, etc. 

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