Membrane proteins unilamellar vesicle interaction

Introduction - Liposomes are vesicles composed of one or more lipid bilayers completely surrounding an internal aqueous space. They are usually composed of phospholipids either in pure form or In combination with other amphipathic molecules such as sterols, long chain bases or acids, or membrane proteins. The structure of liposomes varies from large (0.5->5y) multllamellar vesicles to small ( 300 A) unilamellar vesicles.2,3 More recently, new methods have been reported describing the formation of unilamellar vesicles of intermediate size. >5.6 xhe general properties of liposomes and their interaction with various macromolecules have been described in several reviews. ... 

Winter and co-workers elucidated the interaction between lipidated Ras protein and membrane and investigated the distributiOTi of Ras proteins in membrane microenvironments using two-photon fluorescence microscopy on giant unilamellar vesicles (GUVs) and tapping mode atomic force microscopy (AFM)... 

J, Interaction of Peptides and Proteins with Unilamellar Vesicles Mimicking the Cell Membrane... 

The formation of GUVs in the presence of the various additives under various stress conditions has been studied quantitatively. The presence of CAB in the molten globule state at pH 4 was observed to favor the formation of the larger size of giant unilamellar vesicles. The hydrophobic interaction between vesicles membranes and proteins was found to be a key factor for the formation. 

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