Melted agar

Microscope slides and coverslips Microwave oven, to melt agar and agarose Mortar and pestle Muffle furnace... 

Viable Plate Count A viable cell is defined as one that is able to divide and form a colony. There are two ways of performing a plate count—the spread plate method and pour plate method. With the spread plate method, a volume of no larger than 0.1 mL is spread over the agar surface. With the pour plate method, the sample is mixed with melted agar and poured into a sterile plate. The plate is then incubated until the colonies appear, and the number of colonies is counted. It is important that the number of colonies developing on the plates should be neither too large nor too small. To obtain the... 

Add some of the melted agar to the above supplemented medium and mix gently to yield 0.5% agar medium. 

Cool down the melted agar for 5 min at room temperature before filling the bridges. 

DB Agarose. 100 ml of DB and 0.5 g SeakemME agar (Cat 50010 Lonza). Microwave the suspension to melt agar into DB. Gool at room temperature until the suspension reaches 37°G. Make fresh prior to use. 

Pour 10 ml of 1% melted agar in a 90-mm Petri dish 1 h before assay see Note 9). 

The pour plate The plate colony count isolates bacteria, yeasts, or molds in a quantitative manner. A range of dilutions, usually 10-fold of the sample, is prepared in a sterile diluent and 1 ml of each dilution is added to sterile Petri dishes, mixed with melted agar cooled to 45 °C, and then allowed to solidify. The organisms present in the sample are fixed within the agar gel. Pour plates are then incubated for 2-5 days at 25-37°C, depending on the application. 

Pour plate method Method used to prepare pure cultures using serial dilutions, each of which is mixed with melted agar and poured into a sterile Petri plate. 

Attempts to enhance the low stability by supporting the enzyme on low melting agar has been carried out successfully [51]. Supercritical carbon dioxide was also used as solvent/reagent for semi-purified phenylphosphate carboxylase extracted from T. aromatica [52]. 

Microwave ovens and autoclaves in the lab require certain precautions. Accidents have occurred involving their use (e.g., to melt agar or bactoagar stored in bottles... 

A biotechnological synthesis has also been demonstrated to be possible [58]. Thauera aromatica bacteria can use phenol as the only source of carbon under anaerobic conditions phenol is eventually converted into CO2 and water. The first step of the degradation path is the carboxylation of phenolphosphate to 4-hydroxybenzoic add which is then dehydroxylated to benzoic add [59] (Scheme 1.4). The carboxylation of phenol is carried out by a phenolcarboxylase enzyme, a new type of lyase [60]. The isolation of the enzyme from the cytoplasmic portion of the cell allows its use in vitro. In order to extend the lifetime of the enzyme, its supported form on low melting agar can be used [61]. Cut-off membranes (that allow the passage of macromolecules of a given size) [58] can be... 

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