Pollen germination Media

Fig. 3. Lightmicroscope radioautographs ofintact tubes from pollen germinated 4 h in a medium containing [14C]-proline with or without the proline analogue, azetidine-2-carboxylic acid (AZC). A = 1.0 mM AZC, B = 0.1 mM AZC, C = 0.01 mM AZC, D = 0.001 mM AZC, E = Control. From Dashek and Mills (50), with permission. 

Procedure Pollen develops on the nutrition medium, forming pollen tube (Fig. 3). The nutrient medium for pollen was 10% sucrose solution and tested compounds were also dissolved in 10% sucrose. The pollen develops till the formation of pollen tube that lasts from 2-3 h (fresh collected and one week stored pollen) to 24 h (stored > 1 week, but < 1.5-2 months). All experiments were done at room temperature 20-22 °C. The growth occurred in the solution studied (0.05 ml = 1 drop) on the slides (object glasses) put on wet paper in Petri dishes. Five ml of water was added to the bottom of every dish and 4-5 dishes with the slides were used per treatment. Using light microscope, we determined the microspores germination (%) 2-24 h after moistening. The number of developed pollen tubes was counted. 

Cheng, C.H. and McComb, J.A. (1992). In vitro germination of wheat pollen on raffinose medium. New Phytologist 120 459-462. 

Germinate 20 mg fresh weight lots of Lilium longiflorum, cv. Ace pollen" in Petri dishes containing 10 mL medium supplemented with 0.1 p i[14C]-proline (pro) for 4 h at 27 2°C. 

Wash germinated pollen with medium lacking [14C]-pro and count washes via liquid scintillation. 

The procedures of harvest, storage and in vitro germination of pollen from Olea euroipaea L. cv. Marteno have been described in a previous work 4. Mature or 24 h germinated pollen (2 g) were incubated at 27 in 40 ml of a medium containing 10% sucrose, 0.2 mM CaSO, 2 mM K SO, 0.01% H BO, ... 

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